| 白花泡桐叶柄愈伤组织再生植株的诱导与培养 |
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| 引用本文： | 曲金柱,崔波,马杰,张仙云,范春丽.白花泡桐叶柄愈伤组织再生植株的诱导与培养[J].信阳师范学院学报(自然科学版),2006,19(4):407-410. |
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| 作者姓名： | 曲金柱 崔波 马杰 张仙云 范春丽 |
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| 作者单位： | 1. 郑州师范高等专科学校,生物技术研究所,河南,郑州,450044
2. 河南省科学院,生物研究所,河南,郑州,450008 |
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| 基金项目： | 河南省科技攻关项目(0524050005) |
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| 摘 要： | 为提高白花泡桐繁殖效率,防止丛枝病的传播,保持优良种性,利用愈伤组织再生技术对其增殖.研究表明:叶柄产生愈伤组织最适培养基为MS 2.0 mg/L BA 0.2 mg/L NAA 0.05 mg/L2,4-D;诱导芽分化最适培养基为1/2MS 2 000 mg/L脯氨酸,可使诱导分化率达27%;增殖继代最适培养基为B5 1.0 mg/L BA 0.1 mg/L NAA,增殖系数达6.79;生根最适培养基为1/2MS 0.1 mg/L IBA 0.1 mg/L NAA,生根率达100%.
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| 关 键 词： | 白花泡桐 愈伤组织 再生植株 |
| 文章编号： | 1003-0972（2006）04-0407-04 |
| 收稿时间： | 2005-12-20 |
| 修稿时间： | 2005年12月20 |
Plant Regeneration from Petiole Calli of Paulownia fortunei |
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| QU Jin-zhu,CUI Bo,MA Jie,ZHANG Xian-yun,FAN Chun-li.Plant Regeneration from Petiole Calli of Paulownia fortunei[J].Journal of Xinyang Teachers College(Natural Science Edition),2006,19(4):407-410. |
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| Authors: | QU Jin-zhu CUI Bo MA Jie ZHANG Xian-yun FAN Chun-li |
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| Institution: | 1. Institute of Biotechnology, Zhengzhou Teachers College,Zhengzhou 450044, China; 2. Institute of Biology, Henan Academy of Sciences,Zhengzhou 450008,China |
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| Abstract: | For developing propagation,preventing the spreading of Paulownia Witches'-broom(PaWB) and holding excellent variety,the technique of plant regeneration from petiole calli of Paulownia fortunei and rapid propagation was studied.The result showed that MS BA2.0 mg/L NAA0.2 mg/L 2.4-D0.05 mg/L was the best culture medium of callus induction.The suitable medium for regeneration was 1/2MS 2 000 mg/L proline.The regeneration frequency can be 27%.The best culture medium of propagation was B_5 BA1.0 mg/L NAA0.1 mg/L.The propagation quotient was 6.79.(1/2MS) IBA0.1mg/L NAA0.1mg/L was the best culture medium of inducing the rhizogenesis .The rate of rhizogenesis was 100%. |
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| Keywords: | Paulownia fortunei callus plant regeneration |
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