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| 葱莲凝集素基因的克隆 | |
| 引用本文： | 庞永珍,姚剑虹,申国安,唐克轩,谈锋. 葱莲凝集素基因的克隆[J]. 西南师范大学学报(自然科学版), 2002, 27(3): 433-436 |
| 作者姓名： | 庞永珍 姚剑虹 申国安 唐克轩 谈锋 |
| 作者单位： | 1. 复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海,200433;西南师范大学生命科学学院,重庆,400715 2. 复旦大学生命科学学院遗传学研究所遗传工程国家重点实验室,上海,200433 3. 西南师范大学生命科学学院,重庆,400715 |
| 摘 要： | RACCE－PCR技术从葱莲叶片中克隆出葱莲凝集素的全长cDNA，通过比较葱莲同其它石蒜科植物的凝集素基因序列和推测的氨基酸序列，发现葱莲凝集素基因编码一具有信号肽的前体蛋白，葱莲凝集素同其它石蒜科植物凝集素一样为具有3个甘露糖专一结合盒的凝集素。 |
| 关 键 词： | cDNA 凝集素基因 RACE－PCR 葱莲 基因克隆 石蔚料 氨基酸序列 基因编码 |
Molecular Cloning of Lectin Gene from Zephyranthes Candida |
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| PANG Yong zhen ,,YAO Jian hong ,SHEN Guo an ,TANG Ke xuan ,TAN Feng State Key Laboratory of Genetic Engineering. Molecular Cloning of Lectin Gene from Zephyranthes Candida[J]. Journal of southwest china normal university(natural science edition), 2002, 27(3): 433-436 | |
| Authors: | PANG Yong zhen YAO Jian hong SHEN Guo an TANG Ke xuan TAN Feng State Key Laboratory of Genetic Engineering |
| Affiliation: | PANG Yong zhen 1,2,YAO Jian hong 1,SHEN Guo an 1,TANG Ke xuan 1*,TAN Feng 2 1 State Key Laboratory of Genetic Engineering,Institute of Genetics,School of Life Sciences,Fudan University,Shanghai 200433,China, 2 School o |
| Abstract: | The full length cDNA of Zephyranthes candida lectin gene was cloned from leaves by using RACE PCR. Through comparative analysis of Zephyranthes candida lectin gene and its deduced amino acid sequence with those of other Amaryllidaceae species, Zephyranthes candida lectin gene was found to encode a precursor lectin with signal peptide. Zephyranthes candida lectin was a mannose binding lectin with three mannose binding boxes like lectins from other species of Amaryllidaceae. |
| Keywords: | cDNA lectin gene RACE PCR Zephyranthes candida |
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