包囊游仆虫纤毛器微管在不同生理状态下的分化

应用FLUTAX染色及荧光显微术研究了包囊游仆虫的皮层微管胞器及其形成包囊和脱包囊过程中结构的分化：（1）细胞无性分裂过程中，棘毛基部的毛基体由紧密聚集状态逐渐分离、瓦解和消失；背纤毛基体逐渐膨大，基体内的周围微管散开成梅花形，之后在相应皮层区发生由颗粒组成的条带形原基.（2）细胞形成包囊时，口围带、波动膜和额腹横棘毛等微管胞器经历了部分去分化的过程，伴随着细胞体的凝缩，其纤毛器相互聚集，定位于球形体包囊细胞腹面；背纤毛按原有模式排列，位于包囊细胞背面.（3）脱包囊过程中，细胞吸水膨大，细胞体分化成变形虫状并显示一致的弱荧光，但未见微管胞器的荧光图像；细胞通过包囊壁背面小孔脱囊而出，恢复成为正常形态的纤毛虫；此后，在细胞脱包囊后残留的包囊壁背壁尚保存有部分背纤毛的痕迹.根据所得结果推测,形态发生中，纤毛器微管对新结构的形成可能有物质联系或物质贡献；脱包囊时，细胞成变形虫状，其微管结构可能发生了解聚和再分化的过程.

Ciliature Microtubule Differentiation of Euplotes encysticus in Different Physiological Conditions(Chinese)

The ciliature microtubules of Euplotes encysticus and their structural differentiation during asexual division,encystment and excystment were investigated by using FLUTAX staining and fluorescence microscopic techniques.(1) During morphogenesis,the close gathered kinetosomes of FVTcirri become separated from each other,disintegrate and disappear gradually.The base bodies of dorsal cilia expand gradually and in the mean time the peripheral microtubules in basal bodies apart gradually from each other forming a plum blossom-like arrangement.Afterwards,the bands of granular cilia-anlagen occur in the corresponding cortical generating regions.(2) During encystment,AZM,PM,FVT-cirri and other organelles undergo a partial dedifferentiation process,i.e.with the condense of cell body,ciliatures aggregate each other at the ventral part of the spherical cyst cell body and the dorsal cilia arrange at the dorsal part with original pattern.(3) During excystment,the cell body swells as water is absorbed into the cell and becomes amoeba-like in shape.During this developmental stage,although weak fluorescence could be detected all over the cell,the fluorescence image of microtubules was never observed.The differentiated cell gets out of the cyst through a dorsal small pore of cyst wall and becomes a morphologically normal ciliate.Some tracks of dorsal cilia could be found at the dorsal part of the cyst wall left over during excystment.Based on the above results,following conclusions were suggested: during morphogenesis,the microtubules of ciliatures may have some contributions to the construction of new structures of the ciliate;during excystment,the microtubular structures have a further differentiating process,accompanying the differentiation of ameba-like shaped cell body.After these developmental events,all microtubules may go through a futher step of assembling and morphogenesis.