吡喃糖氧化酶电极对葡萄糖和木糖的响应特征

采用戊二醛交联方法，将葡萄糖氧化酶(GOD)和吡喃糖氧化酶分别固定在醋酸纤维素载体膜上，制备电流型电化学酶电极。在SBA双电极分析仪器上安装葡萄糖氧化酶电极和吡喃氧化糖氧化酶电极，分别对不同浓度的葡萄糖、木糖及葡萄糖木糖混合样品进行测试，以葡萄糖氧化酶电极为对照，研究吡喃糖氧化酶电极对葡萄糖、木糖的响应特征。结果表明，吡喃糖酶电极对葡萄糖和木糖都具有良好的响应特征。葡萄糖测定的精密度（RSD）为1.10%（n=10），在10~1 000 mg/L范围内有良好的线性相关性（R=0.999 0）；木糖测定的RSD为0.80%（n=10），在10~1 600 mg/L范围内有较好的线性相关性（R=0.999 8）；在葡萄糖木糖混合液测定时，吡喃糖氧化酶电极呈现明显的双底物催化特征，在一定的浓度范围内，对葡萄糖和木糖的响应具有线性相关性。

Response characteristics of pyranose oxidase electrode to glucose and xylose

We respectively immobilized pyranose oxidase (PROD) and glucose oxidase (GOD) on the membrane of cellulose acetate with glutaralde hydecros slinker and prepared amperometric electrochemical enzyme electrodes. We respectively detected different concentrations glucose, xylose and their combinations. We addressed the response characteristics of PROD electrode to glucose and xylose with GOD electrode as a control. Results show that PROD electrode has better response characteristics to both glucose and xylose.The reproducibility ( RSD) is 1.10%(n=10) for glucose and 0.80% (n=10）for xylose.Better linear relationship is 0.9990 in the scope of 10~1 000 mg/L for glucose, and 0.9998 in the scope of 10~1 600 mg/L for xylose. PROD electrode exhibits dominant double substrate catalytic characteristics for the combination of glucose and xylose, whose response to glucose and xylose has linear relationship within a certain concentration range.