| 细胞膜淀粉酶的纯化和分析(英文) |
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| 引用本文： | 徐亮,ANDERSON Kevin. 细胞膜淀粉酶的纯化和分析(英文)[J]. 西南师范大学学报(自然科学版), 2004, 0(3) |
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| 作者姓名： | 徐亮 ANDERSON Kevin |
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| 作者单位： | 重庆工商大学环境与生物工程学院 重庆400067(徐亮)，密西西比州立大学文理学院 美国(ANDERSON Kevin) |
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| 摘 要： | 一种Ruminobacteramylophilus70细胞膜联的新支连淀粉酶被4%TritonX 100提取.其新支连淀粉酶活性存在于70%的硫酸铵沉淀组分中.通过等电聚焦纯化,根据SDS凝胶电泳测定,其新支连淀粉酶的分子量是91 2kDa,其等电点是5 8～5 9.根据另一种淀粉酶活性凝胶电泳的方法测定,其新支连淀粉酶的分子量大约是92 6kDa.在R.amylophilus70细胞中存在着两种类型的淀粉酶(可溶性淀粉酶和膜联淀粉酶,比如新支连淀粉酶)共同承担分解淀粉的作用.
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| 关 键 词： | 细胞膜联新支连淀粉酶 等电点 分子量 |
Purification and Analysis of Membrane-associated Neopullulanase of Ruminobacter amylophilus 70 |
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| XULiang,ANDERSON Kevin. School of Environment and Biological Engineering,Chongqing Technology and Business University,Chongqing ,China,. School of Science and Art,Mississippi State University,USA.. Purification and Analysis of Membrane-associated Neopullulanase of Ruminobacter amylophilus 70[J]. Journal of southwest china normal university(natural science edition), 2004, 0(3) |
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| Authors: | XULiang ANDERSON Kevin. School of Environment Biological Engineering Chongqing Technology Business University Chongqing China . School of Science Art Mississippi State University USA. |
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| Affiliation: | XULiang~1,ANDERSON Kevin~21. School of Environment and Biological Engineering,Chongqing Technology and Business University,Chongqing 400067,China,2. School of Science and Art,Mississippi State University,USA. |
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| Abstract: | |
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| Keywords: | membrane-associated neopullulanase isoelectric point molecular weight |
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