Effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer (MCF-7) tumors implanted in ovariectomized nude mice |
| |
Authors: | Qian Wu NianZu Jin Jing Yu RenChen Zhao ZePing Yu ShanLei Qiao XiaoHe Lu ChunWen Zhang |
| |
Institution: | (1) Department of Hygienic Analysis and Detection, School of Public Health, Nanjing Medical University, Nanjing, 210029, China;(2) Research Institute of General Surgery, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing, 210002, China;(3) Department of Pharmacy, Nanjing General Hospital of PLA, Nanjing Military Command, Nanjing, 210002, China |
| |
Abstract: | In this study, we explored the effects of soy isoflavone extracts on the growth of estrogen-dependent human breast cancer
(MCF-7) tumors implanted in ovariectomized athymic mice. The ovariectomized athymic mice were implanted with MCF-7 cells.
They were fed with low, moderate and high doses of soy isoflavone extracts, at dietary concentrations of 6.25, 12.5 and 25
g/kg, respectively. The expression of ki-67 was detected by immunohistochemistry. The pS
2 expression in tumors was analyzed by real-time PCR. Estrogen level in the serum was measured by chemiluminescence enzyme
immunoassay. Compared with the control group, dietary soy isoflavone extracts had a significant stimulatory effect on MCF-7
tumor growth in mice (P < 0.05). The ki-67 and pS
2 mRNA expressions in tumors were significantly increased by 6.25 and 12.5 g/kg dose of soy isoflavone extracts (P < 0.05). And, estrogen level in serum of 6.25 and 12.5 g/kg dose groups was higher than that of control group (P > 0.05). In conclusion, in the tested dietary concentration range soy isoflavone extracts had a stimulatory effect on tumor
growth. 6.25 and 12.5 g/kg doses of soy isoflavone extracts can increase the cell proliferation in tumors and induce estrogen-responsive
pS2 expression.
Supported by National Natural Science Foundation of China (Grant No. 30572133) |
| |
Keywords: | soy isoflavone extracts breast cancer nude mice MCF-7 estrogen ki-67 pS 2 |
本文献已被 SpringerLink 等数据库收录! |
| 点击此处可从《中国科学通报(英文版)》浏览原始摘要信息 |
| 点击此处可从《中国科学通报(英文版)》下载免费的PDF全文 |
|