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微通道内葡萄糖分子荧光检测的研究
引用本文:白忠臣,秦水介.微通道内葡萄糖分子荧光检测的研究[J].四川大学学报(自然科学版),2007,44(5):1069-1072.
作者姓名:白忠臣  秦水介
作者单位:贵州大学光电子技术及应用重点实验室,贵阳,550025
基金项目:国家自然科学基金;教育部高等学校优秀青年教师教学科研奖励计划;教育部留学回国人员科研启动基金;高等学校博士学科点专项科研项目
摘    要:本文介绍一种在微米通道内对经荧光标记的葡萄糖分子的荧光检测新方法,该方法成功地实现了在微通道内对葡萄糖分子的荧光检测.实验验证了用荧光素纳标记葡萄糖分子的可行性;分析了激发光波长和溶液的酸碱度对实验结果的影响.结果表明:在微通道内可有效地识别荧光素纳标记的葡萄糖分子.基于此研究可以在生物分析、生物检测等领域找到新的应用.

关 键 词:荧光检测  葡萄糖分子  微通道  荧光素纳
文章编号:0490-6756(2007)05-1069-04
收稿时间:4/8/2007 12:00:00 AM
修稿时间:2007-04-08

Study on fluorescent detection for glucose molecule in microchannels
BAI Zhong-chen and QIN Shui-jie.Study on fluorescent detection for glucose molecule in microchannels[J].Journal of Sichuan University (Natural Science Edition),2007,44(5):1069-1072.
Authors:BAI Zhong-chen and QIN Shui-jie
Institution:Laboratory for Photoelectric Technology and Application, Guizhou University,Laboratory for Photoelectric Technology and Application, Guizhou University
Abstract:A new method of the fluorescent detection is introduced to use for detecting glucose molecule marked by fluorescence in microchannels. The fluorescent detection for glucose molecule is successfully realized in microchannels. The experimental results show that it is feasible to use sodium fluorescein marking the glucose molecule. At the same time, The experiment analyzes that experimental results have been influenced by wavelength of exciting light and acidity and basicity of solution. It is concluded that the glucose molecule marked with sodium fluorescein can be identified effectively in microchannels in this experiment. The new technology based on the study will lead to new application in bioanalysis and biological detection.
Keywords:Fluorescent detection  glucose molecule  microchannel  sodium fluorescein
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