| 吉林省主推中晚熟期玉米品种DNA指纹库的构建 |
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| 引用本文： | 付玉芹,隋光磊,江源,王丕武,关淑艳,刘树英,胡欣.吉林省主推中晚熟期玉米品种DNA指纹库的构建[J].吉林大学学报(理学版),2008,46(6):1221-1224. |
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| 作者姓名： | 付玉芹 隋光磊 江源 王丕武 关淑艳 刘树英 胡欣 |
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| 作者单位： | 1. 吉林农业大学 生命科学学院, 长春 130118; 2. 中国人民解放军总后勤部 军需装备研究所， 北京 100010 |
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| 基金项目： | 吉林省科技厅科研项目基金，吉林农业大学博士启动基金 |
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| 摘 要： | 以吉林省中晚熟期的43个玉米品种为材料, 从玉米胚中提取基因组DNA并利用10对简单重复序列(SSR)引物进行扩增, 扩增产物经质量分数为8%的聚丙烯凝胶电泳检测, 获得了清晰稳定的图谱, 初步建立了吉林省中晚熟期玉米43个品种的DNA指纹库.
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| 关 键 词： | 玉米 简单重复序列 DNA指纹 |
| 收稿时间： | 2008-03-14 |
Establishment of DNA Fingerprinting Database of Dominant Middle-late Mature Maize Hybrids in Jilin Province |
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| FU Yu-qin,SUI Guang-lei,JIANG Yuan,WANG Pi-wu,GUAN Shu-yan,LIU Shu-ying,HU Xin.Establishment of DNA Fingerprinting Database of Dominant Middle-late Mature Maize Hybrids in Jilin Province[J].Journal of Jilin University: Sci Ed,2008,46(6):1221-1224. |
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| Authors: | FU Yu-qin SUI Guang-lei JIANG Yuan WANG Pi-wu GUAN Shu-yan LIU Shu-ying HU Xin |
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| Institution: | 1. College of Life Science, Jilin Agricultural University, Changchun 130118, China;2. Institute of the Quartermaster, General Logistics Department of the PLA, Beijing 100010, China |
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| Abstract: | Forty three dominant middle late mature maize hybrids in Jilin Province were selected in this study, from which DNA was extracted and
amplified with 10 pairs of simple sequence repeat (SSR) primers, the products were detected with 8% polyacrylamide gelelectrophoresis. Clear and stable profiles of 43 samples were gained and the DNA fingerprinting database of 43 middle late mature maize hybrids was established. |
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| Keywords: | maize simple sequence repeat (SSR) DNA fingerprinting |
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