人工催产时泥鳅(Misgurnus anguillicaudatus)尾部神经分泌系统单胺荧光和乙酰胆碱酯酶活性的变化

在人工催产过程中,我们用单胺荧光结合乙酰胆碱醋酶(AChE)组化方法对泥鳅(Misgurnus anguillicaudatus)尾部神经分泌系统进行了研究。给泥鳅注射LRH-A(0.05微克/克)12小时后,尾部神经分泌系统中单胺荧光强度明显比催产前增强,但神经分泌细胞的AChE活性却明显降低;当注射LRH-A 24小时和48小时后,单胺荧光强度减弱,而AChE活性却明显增强。

THE MONOAMINERGIC INNERVATION AND ACETYLCHOLINESTERASE ACTIVITY OF THE CAUDAL NEUROSECRETORY SYSTEM OF Misgurnus Anguillicaudatus IN RELATION TO ARTIFICIALLY INDUCED SPAWNING

During the artificially induced spawning, the caudal neurosccretory system of Misgurnus anguillicaudatus (Cantor) was studied by means of monoamine fluorescencc reaction combined with a histochemical method for acetylcholinesterase (ACHE). Twelve hours alter LRH-A (0.05ug/g) was injected, the fluorescence of beaded fibers and varicosities contiguous to the caudal neurosecretory cells were increased markedly compared to the control group, but AChE activity of the neurosecretory cells was reduced significantly. 24 and 48 hours after injections of LRH-A, both the intensity of fluorescence and the number of varicosities in close cotact with the caudal neurosecretory perikarya were reduced. However, AChE activity was increased markedly. A correlation between catecholamines, AChE activity and reproductive processes is discussed.