| Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets( Cl) mutant |
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| 作者姓名： | ZHENGLeiying ZHUXudong |
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| 作者单位： | [1]NationalKeyLaboratioryofPlantMolecularGenetics,ShanghaiInstituteofPlantPhysiologyandEcology,ChineseAcademyofSciences,Shanghai200032,China [2]ChianNationalRiceResearchInstitute,Hangzh |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | The rice clustered spikelets (Cl) mutant exhibits a phenotype that most of branch apical have 2-3 spikelets clustered together,SEM (scanning electron microscope )observation suggested that the Cl gene controlled branch apical development,and influenced the terminal spikelets elongation,The spikelet number was reduced in mutant,indicating that Cl may also have an effect on spikelet number,To map Cl locus,two F2 mapping populations derived from the crosses between the Cl and ZhongHua11,and Cl and ZheFu802 were constructed ,respectively,The Cl locus was roughly mapped between two CAPS markers CK0214 and SS0324,A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl12560,with genetic distances of 0.2 and 2.1 cM,respectively ,Then we found a PAC conting spanning Cl locus,the region was delimited to 196 kb.This results was useful for cloning of the Cl gene,Allelism test demonstrated that Cl was allelic to Cl2 another rice clustered spikelets mutant.
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| 关 键 词： | 水稻 簇生小穗突变体 分子标记 基因图遗传分析 植物形态 SEM |
| 收稿时间： | 2002-12-24 |
Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets (Cl) mutant |
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| Leiying Zheng, Xudong Zhu, Qian Qian, Zhong Zhao, Jianjun Zhang, Xiaohe Hu, Hongxuan Lin and Da Luo.Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets( Cl) mutant[J].Chinese Science Bulletin,2003,48(6):559-562. |
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| Authors: | Leiying Zheng Xudong Zhu Qian Qian Zhong Zhao Jianjun Zhang Xiaohe Hu Hongxuan Lin and Da Luo |
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| Institution: | (1) National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 200032 Shanghai, China;(2) China National Rice Research Institute, 310006 Hangzhou, China;(3) Crop Institute of Breeding and Cultivation, Shanghai Agricultural Academy, 201106 Shanghai, China;(4) Shanghai Anxin Research Foundation Laboratory, 200032 Shanghai, China |
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| Abstract: | The rice clustered spikelets (Cl) mutant exhib-its a phenotype that most of branch apical have 2-3 spikelets clustered together. SEM (scanning electron microscope) ob-servation suggested that the Cl gene controlled branch apical development, and influenced the terminal spikelets elonga-tion. The spikelet number was reduced in mutant, indicating that Cl may also have an effect on spikelet number. To map Cl locus, two F2 mapping populations derived from the crosses between the Cl and ZhongHua11, and Cl and ZheFu802 were constructed, respectively. The Cl locus was roughly mapped between two CAPS markers, CK0214 and SS0324. A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl2560, with genetic distances of 0.2 and 2.1 cM, respectively. Then we found a PAC contig spanning Cl locus, the region was delim-ited to 196 kb. This result was useful for cloning of the Cl gene. Allelism test demonstrated that Cl was allelic to Cl2, another rice clustered spikelets mutant. |
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| Keywords: | oryza sativa clustered spikelets mutant SEM gene map-ping molecular marker allelism test |
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