苦楝和杉木的种间化感作用研究

摘要：采用正交试验方法和超临界流体萃取法，研究了苦楝叶提取物对杉木的化感作用。生物检测结果显示，与酒精对照相比：萃取参数1、4和7等条件苦楝叶提取物对杉木种子绝对发芽率、绝对发芽势、胚根长、胚轴长、鲜重和干重等发芽指标均表现出不同程度的促进作用，萃取参数2和3等条件苦楝叶提取对除胚根和胚轴外的各发芽指标起促进作用，萃取参数5条件苦楝叶提取物对除鲜重和干重外的各发芽指标起促进作用，萃取参数6条件苦楝叶提取物对除胚根和鲜重外的各发芽指标起促进作用，而萃取参数8条件苦楝叶提取物仅对绝对发芽势起促进作用。同一参数条件三种浓度相比：萃取参数1、4、5和7条件下以100mg/kg浓度苦楝叶提取物处理的对杉木种子各发芽指标促进作用最明显，接着为50mg/kg或200mg/kg浓度处理的；萃取参数2和3条件下以200mg/kg浓度提取物处理的促进作用最强，接着为50mg/kg或100mg/kg浓度处理的。

Study on Allelopathy of Melia azedrach and Chinese-fir

Abstract:Melia azedrach leaves extracts through supercritical fluid extract under orthogonal experiment affected the Chinese fir seeds germination differently. Bioassay results revealed: compared with alcohol control, extracts at parameter 1, 4, 7 stimulated the absolute germination rate and potential, radicle growth, plumular axis growth, fresh weight, dry weight at different levels; extracts at parameter 2, 3 stimulated the all germination index except radicle growth and axis growth; extracts at parameter 6 stimulated all but radicle growth and fresh weight; extract at parameter 8 stimulated only the germination potential. Among the three concentrations at the same parameter, extract of 100mg/kg at parameter 1, 4, 5, 7 stimulated the germination most, the following were 50mg/kg and 200mg/kg; extract of 200mg/kg stimulated most , the following were 50mg/kg and 200mg/kg.