| 展示肿瘤特异性抗原表位的噬菌体在小鼠体内引起的细胞免疫变化 |
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| 引用本文： | 房金波,赵连贵,宋金娜,高瑞娟,王丽.展示肿瘤特异性抗原表位的噬菌体在小鼠体内引起的细胞免疫变化[J].东北师大学报(自然科学版),2005,37(3):69-72. |
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| 作者姓名： | 房金波 赵连贵 宋金娜 高瑞娟 王丽 |
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| 作者单位： | 东北师范大学生命科学学院,吉林,长春,130024;白城市教育学院,吉林,白城,137000 |
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| 基金项目： | 国家自然科学基金，吉林省科技厅国际合作项目 |
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| 摘 要： | 以含有肿瘤特异性抗原表位的杂合型噬菌体(TSPA-M-A1)作为抗原,免疫C57BL/ 6J小鼠,观察免疫4周后小鼠的细胞免疫变化.结果表明:抗原TSPA-M-A1在小鼠体内能够提高T淋巴细胞对ConA刺激的反应性,使NK细胞杀伤活性明显增高,产生了特异性T淋巴细胞(CTL)反应,引起了较强的迟发型超敏反应.这些结果为肿瘤的免疫治疗提供了实验依据.
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| 关 键 词： | 噬菌体展示 肿瘤特异性抗原 细胞免疫 CTL |
| 文章编号： | 1000-1832（2005）03-0069-04 |
| 收稿时间： | 2005-01-23 |
| 修稿时间： | 2005年1月23日 |
Filamentous bacteriophage displayed tumor-specific antigen epitope(MAGE-A1) induced cellular immune in mice |
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| FANG Jin-bo,ZHAO Lian-gui,SONG Jin-na,GAO Rui-juan,WANG Li.Filamentous bacteriophage displayed tumor-specific antigen epitope(MAGE-A1) induced cellular immune in mice[J].Journal of Northeast Normal University (Natural Science Edition),2005,37(3):69-72. |
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| Authors: | FANG Jin-bo ZHAO Lian-gui SONG Jin-na GAO Rui-juan WANG Li |
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| Abstract: | The C57BL/6J mice were immunized with the purified hybrid phage, which displayed the tumor-specific antigen epitope MAGE-A1_(161-169) (TSPA-M-A1). Tested the proliferation of T lymphocytes, NK kill rate, CTL response in the spleen by MTT, incorporating ~3H-TdR and chromium-release assays. The result showed that the proliferation of T lymphocytes increased, the NK activity was enhanced obviously compared with control, elicited MAGE-A1 specific CTL responses in the spleen and delayed-type hypersensitivity(DTH). These observations may provide a new strategy for cancer immunotherapy. |
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| Keywords: | CTL |
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