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| 高效液相色谱法测定硒-甲基硒代半胱氨酸 | |
| 引用本文： | 刘建群,刘芳,张锐,张小平,寇晓莉.高效液相色谱法测定硒-甲基硒代半胱氨酸[J].萍乡高等专科学校学报,2009,26(3). |
| 作者姓名： | 刘建群 刘芳 张锐 张小平 寇晓莉 |
| 作者单位： | 1. 江西中医学院现代中药制剂教育部重点实验室,江西南昌,330004 2. 江西萍乡高等专科学校化工系,江西萍乡,337000 3. 江西川奇药业有限公司,江西南昌,330029 |
| 摘 要： | 建立硒-甲基硒代半胱氨酸(SeMC)测定方法,优选合成工艺,控制成品质量。采用高效液相色谱法(HPLC),测定条件为:色谱柱为HypersilC4(4.6mm×300mm,5μm),流动相为水(用磷酸调至pH2.50)-甲醇(95:5),检测波长235nm,流速1.0ml/min,柱温25℃。结果:硒-甲基硒代半胱氨酸的线性范围为0.08μg～2μg,r=0.9995,高、中、低三种不同浓度的平均加标回收率范围为99.97%～100.62%,RSD为0.53%～0.88%。结论:该法操作便捷,结果准确,适合合成工艺评价和成品质量控制。 |
| 关 键 词： | 硒-甲基硒代半胱氨酸 含量测定 高效液相色谱法 |
Determination of Se-methylselenocysteine by HPLC |
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| Liu Jianqun,Liu Fang,Zhang Rui,Zhang Xiaoping,Kou Xiaoli.Determination of Se-methylselenocysteine by HPLC[J].Journal of Pingxiang College,2009,26(3). | |
| Authors: | Liu Jianqun Liu Fang Zhang Rui Zhang Xiaoping Kou Xiaoli |
| Institution: | 1.Key Laboratory of Modern Preparation of TCM;Ministry of Education;Jiangxi University of Traditional Chinese Medicine;Nanchang 330004;2.Department of Chemistry;Pingxiang College;Pingxiang 337000;3.Chuanqi Pharmaceutical Company;Nanchang 330029;China |
| Abstract: | Objective :To establish the assay of Se-methylselenocysteine for technology and quality control.Methods:A HPLC method was established with the follow measurement conditions.Hypersil C4 column was used with water(adjust pH to 2.50 with phosphoric acid)-MeOH(95:5)as the mobile phase.The content was detected at wavelength of 235nm and at temperature of 25℃.The flow rate was at 1.0ml/min.Results:The calibration curve was linear in the range of 0.08μg-2μg,r=0.9995.The recovery range for three concentrations of h... |
| Keywords: | Se-methylselenocysteine determination HPLC |
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