| 4种抗生素对菘蓝外植体生长的影响 |
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| 引用本文： | 曾建军,孙敏,肖璇,江波. 4种抗生素对菘蓝外植体生长的影响[J]. 西南师范大学学报(自然科学版), 2003, 28(6): 987-990 |
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| 作者姓名： | 曾建军 孙敏 肖璇 江波 |
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| 作者单位： | 1. 西南师范大学,生命科学学院,重庆,400715;井冈山师范学院,生命科学系,江西,吉安,343009
2. 西南师范大学,生命科学学院,重庆,400715 |
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| 摘 要： | 对抗生素卡拉霉素和潮霉素进行菘蓝外植体抗性浓度筛选，结果表明不同外植体具有不同抗性．子叶芽分化阶段，卡拉霉素和潮霉素筛选浓度范围分别为10-50mg／L和5-30mg／L；下胚轴芽分化阶段的筛选浓度分别为15-50mg／L和10-30mg/L；分化苗生根时筛选浓度分别为30mg/L和20mg/L．芽分化阶段宜使用头孢霉素为抗菌剂，但分化苗生根时宜选用羧苄青霉素．
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| 关 键 词： | 抗生素 菘蓝 外植体生长 卡拉雹素 潮霉素 头孢霉素 羧苄青霉素 抗性浓度 板蓝根 药用植物 |
| 文章编号： | 1000-5471(2003)06-0987-04 |
| 修稿时间： | 2003-06-03 |
The Effects of Four Antibiotics On the Explant''s Growth of Isatis indigotica Fort |
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| ZENG Jian-jun. The Effects of Four Antibiotics On the Explant''s Growth of Isatis indigotica Fort[J]. Journal of southwest china normal university(natural science edition), 2003, 28(6): 987-990 |
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| Authors: | ZENG Jian-jun |
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| Affiliation: | ZENG Jian-jun~ |
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| Abstract: | Through screening concentration of kanamycin and hygrimycin from culturing explants of Isatis indigotica Fort, the result showed that resistance concentration is different from different explant. In the stage of shoot regeneration from cotyledon, kanamycin is 10-50 mg/L, hygrimycin is 5-30 mg/L. In the stage of shoot regeneration from hypocotyls, kanamycin is 15-50 mg/L, hygrimycin is 10-30 mg/L. When plantlet grow in root medium, kanamycin is 30 mg/L, hygrimycin is 20 mg/L. In different stages of shoot or root regeneration, the best antibacterial agent is cefotaxime an or carbenicillin respectively. |
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| Keywords: | Isatis indigotica Fort kanamycin hygrimycin cefotaxime carbenicillin resistance concentration |
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