Isolation of candidateR disease resistance genes from rice

Using a polymerase chain reaction (PCR) based method six distinct candidate disease resistant gene (R) homologs from rice have been isolated. The rice sequences are organized into two phylogenetic groups with contrasting genomic organization patterns. The first group, represented by a single sequence, Osh359-1, is more similar to non-riceR sequences than to rice ones and has a simple genomic organization. The second group, represented by Osh359-3, contains the remaining five rice sequences. Osh359-3 consists of a multi-gene family. The members of Osh359-3 family are further found to be clustered together in the genome.