胰蛋白酶修饰兔红细胞对几种豆科植物凝集活性的影响

圆叶舞草，田菁和糙毛假地豆３种豆科植物种子抽提液对正常兔红细胞均表示较弱的凝集活性，但对经胰蛋白酶修饰后的兔红细胞的凝集作用却较强。实验结果表明，兔红细胞经１．７５ＵｍＬ的胰蛋白酶修饰后凝集活性有明显的提高，其中圆叶舞草提高了１２８倍，田菁提高了６４倍，糙毛假地豆提高了８倍。

EFFECTS OF TRYPSIN ON AGGLUTINABILITY OF RABBIT ERYTHROCYTES WITH LECTINS FROM THREE LEGUMES

Three letins of Desmodium Gyroides, Sesbania canabina, and Desmodium heterocarum DC var . strigosum Meeuwen showed lower agglutinability to untreated rabbit erythrocyte. However, their agglutinability were increased by 128, 64, 8 times, respectively, when the rabbit erythrocyte were treated with 1.75 U/mL trypsin. It is analyzed that there are acceptors on the erythrocyte surface, which can combine the erythrocyte with lectins. Some protein or glycopeptide outside the cell membrane block the combination of lecin with its acceptor. Trypsin can partly get rid of such protein or glycopeptide by hydrolyzing peptide chain and increasing agglutinability.