2种非生物胁迫和7种激素对水稻OsAQP基因表达的影响

OsAQP是从cDNA文库中分离的一种新的水稻水通道蛋白基因,本实验室前期利用半定量RT-PCR技术发现该基因的表达与非生物胁迫、植物激素有关.本文采用qRT-PCR方法,检测了干旱、高盐胁迫以及ABA等7种植物激素对OsAQP在幼苗期水稻根和叶中表达的影响,结果显示干旱、高盐以及7种植物激素MeJA,SA,6-BA,GA,IAA,ABA,BR均能不同程度诱导OsAQP基因上调表达,在根中的诱导上调水平显著高于叶,提示该基因功能涉及生长发育、抗逆应答等多种生物学过程,且与水稻的根的关系更为密切.

Effects of Seven Phytohormones and Two Abiotic Stresses on the Expression of Rice OsAQP Gene

OsAQPis a rice aquaporin gene isolated from cDNA library.Using semi-quantitative RT-PCR,it had been identified as a putative response gene for abiotic stresses and plant hormones.In this study,real-time quantitative PCR method was performed to detect the expression pattern of OsAQPin rice seedlings under two abiotic stresses and seven plant hormones treatments.The results show that abiotic stresses,such as drought,high salt and phytohormones,such as MeJA,SA,6-BA,GA,IAA,ABA,BR can induce its expression in leaves and roots,especially in leaves.It can be deduced that OsAQP may involved in rice growth and development,and also abiotic responses which under the regulation of several phytohormones,the function of the gene may relate with the biological functions of rice roots.