| BRC2基元的合成及与靶肽p53(171-192)的相互作用 |
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| 作者单位： | ;1.河南省化工研究所有限责任公司材料与化学工程学院;2.河南工业大学化学化工学院;3.河南工程学院材料与化学工程学院 |
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| 摘 要： | 乳腺癌易感基因2(Breast Cancer Susceptibility Gene 2,BRCA2)是能抑制细胞发生恶变的关键基因.p53蛋白是参与细胞过程的主调控蛋白,p53蛋白要想行使其功能,则需要BRCA2及其产物对p53进行调控.选择BRCA2中的BRC2重复基元,用Fmoc固相合成法合成了BRC2及其3个片段.通过反相高效液相色谱(RPHPLC)和质谱对其进行表征,得到纯度大于90%的目标肽链.利用圆二色谱法初步探究了BRC2及其3个片段与靶肽p53(171-192)的相互作用.结果表明,BRC2的3个肽片段中,片段NEVGFRGFYSAHG在BRC2肽行使其功能中起着主要的作用.
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| 关 键 词： | BRCA2 固相合成 靶肽p53 |
Synthesis of BRC2 and Preliminary Study on the Interaction of BRC2 with Peptide p53(171-192) |
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| Institution: | ,Chemical Research Institute Co.Ltd.of Henan Province,School of Chemistry and Chemical Engineering,Henan University of Technology,School of Material and Chemical Engineering,Henan Institute of Engineering |
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| Abstract: | Breast cancer susceptibility gene 2(BRCA2)is an important tumor suppressor gene.The p53 protein is a master regulatory protein involved in cellular processes,if p53 protein want to exercise its functions,it needs to BRCA2 and its products to its regulation.In this thesis,we selected the repeated motif BRC2 in BRCA2,with Fmoc solid phase synthesis,we synthesized repeated motif BRC2 and its three peptide fragments.Crude peptides isolated and purified by reverse phase high performance liquid chromatography(RP-HPLC),and characterized by mass spectrometry(MS),their purity reached more than 90%.The interaction between p53(171-192)and BRC2 peptides had been investigated by using circular dichroism(CD)spectrum.The results showed that:fragment NEVGFRGFYSAHG plays a major role in the exercise of BRC2 functions. |
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| Keywords: | BRCA2 solid phase synthesis p53(171-192) |
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