植物生长物质对牛膝生长及主要药用成分积累的影响

采用不同植物生长物质组合对牛膝幼苗进行喷施处理和植物化学分析相结合的方法,研究植物生长物质对牛膝生长及其主要药用成分合成的影响.结果表明:1.0mg·L-1 IBA+1.0mg·L-1 MJ(T4)协同处理最有利于牛膝地下部分生物量的增加;1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)协同处理最有利于牛膝根中齐墩果酸的积累;1.0mg·L-1 IBA+2.0mg·L-1 NAA(T2)和1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)处理最有利于牛膝根中蜕皮甾酮的积累.综合考虑各项指标,1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)处理无论是对牛膝的生长还是主要药用成分三萜皂苷和蜕皮甾酮的积累均比对照有显著性提高,最有利于牛膝药材产量和品质的提高.

Effects of plant growth substance on Growth and Major Medicinal Components Accumulation of Achyranthes bidentata BI

The effect of plant growth substance on the growth and major medicinal components of Achyranthes bidentate Bl.was investigated by spraying cultured seedlings with different combinations of exogenous hormones and phytochemical method.The results showed that 1.0mg·L-1 IBA+1.0mg·L-1 MJ(T4)coordination treatment significantly promoted underground biomass increasing in A bidentata.1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)coordination treatment was benefit for oleanolic acid accumulation in root,whereas 1.0mg·L-1 IBA+2.0mg·L-1 NAA(T2)and 1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)treatment also significantly promoted ecdysterone accumulation in roots.Taking into account of each index in this experiment,1.0mg·L-1 IBA+1.0mg·L-1 6-BA(T3)treatment was advantageous to growth,the oleanolic acid and ecdysterone total contents increasing in root of A bidentate,and are beneficial to the improvement the output and quality of A bidentate crude drug.