| 乙肝病毒hbx基因诱导斑马鱼胚胎发育背部化畸形 |
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| 引用本文： | 邓娅琦,黄熠,李正,黄林,姚少华,杨寒朔,胡火珍.乙肝病毒hbx基因诱导斑马鱼胚胎发育背部化畸形[J].四川大学学报(自然科学版),2009,46(5):1488-1492. |
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| 作者姓名： | 邓娅琦 黄熠 李正 黄林 姚少华 杨寒朔 胡火珍 |
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| 作者单位： | 1. 四川大学生命科学学院医学细胞生物学教研室,成都,610041
2. 四川大学生命科学学院华西医学院华西医院肿瘤生物治疗国家重点实验室,成都,610041 |
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| 摘 要： | 为了研究HBX对胚胎发育的影响，从含有HBX基因的质粒上转录HBX mRNA，显微注射入斑马鱼早期胚胎，在bud期进行表型观察，并用全胚胎原位杂交法检测HBX对斑马鱼胚胎早期发育的影响，用脊索标记基因ntl和骨骼肌标记基因MyoD作为探针检测胚胎发育背部化的特征表型。首次发现HBX的表达引起斑马鱼胚胎早期发育背部化畸形，HBX过量表达的胚胎背部化表型与早期胚胎wnt缺失表达导致的胚胎发育背部化表型一致，HBX可能通过激活wnt/β-catenin信号通路的表达和调控，造成胚胎发育背部化的畸形。
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| 关 键 词： | 乙肝病毒X基因 显微注射 斑马鱼 背部化 |
| 收稿时间： | 4/1/2008 12:00:00 AM |
| 修稿时间： | 5/5/2008 12:00:00 AM |
hbx expression inciduces the dorsalization of zebrafish embryo |
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| DENG Ya-Qi,HUANG Yi,LI Zheng,HUANG Lin,YANG Han-Shuo,YAO Shao-Hua and HU Huo-Zhen.hbx expression inciduces the dorsalization of zebrafish embryo[J].Journal of Sichuan University (Natural Science Edition),2009,46(5):1488-1492. |
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| Authors: | DENG Ya-Qi HUANG Yi LI Zheng HUANG Lin YANG Han-Shuo YAO Shao-Hua and HU Huo-Zhen |
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| Institution: | The Medicine-Biology Laboratory of College of Life Sciences, Sichuan University;The Medicine-Biology Laboratory of College of Life Sciences, Sichuan University;The Medicine-Biology Laboratory of College of Life Sciences, Sichuan University;The Medicine-Biology Laboratory of College of Life Sciences, Sichuan University;State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, College of Life Sciences, Sichuan University;State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, College of Life Sciences, Sichuan University;The Medicine-Biology Laboratory of College of Life Sciences, Sichuan University |
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| Abstract: | |
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| Keywords: | Hepatitis B virus (HBV) microinjectin zebrofish dorsalization |
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