| HCV多表位抗原基因的表达及其在小鼠中诱导的抗体应答研究 |
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| 引用本文： | 王子来,陈丽珊,李琦涵,陈肖楚,程帆,任大明. HCV多表位抗原基因的表达及其在小鼠中诱导的抗体应答研究[J]. 复旦学报(自然科学版), 2004, 43(2): 163-168,174 |
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| 作者姓名： | 王子来 陈丽珊 李琦涵 陈肖楚 程帆 任大明 |
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| 作者单位： | 复旦大学,生命科学学院,遗传学研究所,遗传工程国家重点实验室,上海,200433;中国医学科学院,昆明生物学研究所,昆明,650118 |
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| 基金项目： | 云南省省院省校合作基金资助项目 |
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| 摘 要： | 丙型肝炎病毒(HCV)是引起病毒性非甲非乙肝炎的主要病原．全世界约有1．7亿HCV的感染者，我国估计占4000万以上．发展有效的丙型肝炎疫苗刻不容缓．构建了3段串联重复的丙型肝炎病毒多表位抗原基因3如，将其克隆到His融合表达载体pET-28b( )上后在大肠杆菌BL21(DE3)中得到了高效表达．3PCX经Ni^2 -NTA—agarose柱纯化后免疫BALB／c小鼠，诱发了高水平的抗体免疫应答．
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| 关 键 词： | 丙型肝炎 疫苗 多表位抗原 免疫反应 |
| 文章编号： | 0427-7104(2004)02-0163-06 |
Expression of a Multiple-epitope Antigen of Hepatitis C Virus and Study of Its Antibody Immune Responses in Mice |
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| WANG Zi-lai. Expression of a Multiple-epitope Antigen of Hepatitis C Virus and Study of Its Antibody Immune Responses in Mice[J]. Journal of Fudan University(Natural Science), 2004, 43(2): 163-168,174 |
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| Authors: | WANG Zi-lai |
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| Affiliation: | WANG Zi-lai~ |
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| Abstract: | Hepatitis C virus(HCV) is the major causative agent of non-A, non-B hepatitis throughout the world. It is estimated that about 170 million people of the world have been infected with HCV and about 40 million of them are Chinese. There is a pressing need to develop an effective vaccine against HCV in order to combat this world wide public health threat. This work constructed a three tandem repeats of a mutlple-epitope antigen gene called 3 pcx and then cloned it into the expression vector pET-28b(+) to express it as hexahistine-tagged protein in Escherichia coli BL21(DE3). After purified by Ni~(2+)-NTA-agarose column, the protein was used to immunize BALB/c mice and it elicited strong antibodies responses. |
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| Keywords: | HCV vaccine multiple-epitope antigen immune responses |
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