| Firing patterns of long-term cultured neuronal network on multi-electrode array |
| |
| 作者姓名： | LI Xiangning ZHOU Wei LIU Man ZENG Shaoqun LUO Qingming |
| |
| 作者单位： | The Key Laboratory of Biomedical Photonics of Ministry of Education-Wuhan National Laboratory for Optoelectronics， Huazhong University of Science and Technology， Wuhan 430074, China |
| |
| 基金项目： | Supported by National Natural Science Foundation of Chinafor Distinguished Young Scholars (Grant No .60025514)，Joint Research Fundfor O-verseas Chinese Young Scholars (Grant No .30328014)，Major Programof Science and Technology Research of Ministry of Education (Grant No .10420) |
| |
| 摘 要： | Spontaneous neuronal activity plays an important role in the development and plasticity of brain. To explore the developmental changes in the firing pattern of the neuronal networks in vitro, the hippocampal neurons were cultured on the multi-microelectrode array dish for over 14 weeks and the spontaneous activity was recorded. The results showed that random firing was observed in the 1st week and transformed into synchronized activity after two weeks, then tightly synchronized activity appeared in week 2 to 7 and finally the activities transformed into the random firing pattern. These results suggested three stages in the long-term development of neuronal network in vitro: the stage for connection, the stage of synchronized activity and the mature stage. Synchronized firing shown by spontaneous activity was an important phenomenon in high density cultured neuronal network and transformed patterns during development.
|
| 关 键 词： | neuronal networks development multi-electrode array cultured neuron. |
Firing patterns of long-term cultured neuronal network on multi-electrode array |
| |
| LI Xiangning,ZHOU Wei,LIU Man,ZENG Shaoqun,LUO Qingming.Firing patterns of long-term cultured neuronal network on multi-electrode array[J].Progress in Natural Science,2006,16(12):1337-1342. |
| |
| Authors: | LI Xiangning ZHOU Wei LIU Man ZENG Shaoqun LUO Qingming |
| |
| Abstract: | Spontaneous neuronal activity plays an important role in the development and plasticity of brain. To explore the developmental changes in the firing pattern of the neuronal networks in vitro, the hippocampal neurons were cultured on the multi-microelectrode array dish for over 14 weeks and the spontaneous activity was recorded. The results showed that random firing was observed in the 1st week and transformed into synchronized activity after two weeks, then tightly synchronized activity appeared in week 2 to 7 and finally the activities transformed into the random firing pattern. These results suggested three stages in the long-term development of neuronal network in vitro: the stage for connection, the stage of synchronized activity and the mature stage. Synchronized firing shown by spontaneous activity was an important phenomenon in high density cultured neuronal network and transformed patterns during development. |
| |
| Keywords: | neuronal networks development multi-electrode array cultured neuron |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《自然科学进展(英文版)》浏览原始摘要信息 |
| 点击此处可从《自然科学进展(英文版)》下载免费的PDF全文 |
