| 供氧对紫苏细胞生长和花色素生产影响的初步试验 |
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| 引用本文： | 钟建江,吉田敏臣.供氧对紫苏细胞生长和花色素生产影响的初步试验[J].华东理工大学学报(自然科学版),1994,20(6):769-772. |
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| 作者姓名： | 钟建江 吉田敏臣 |
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| 作者单位： | 华东理工大学生物化学工程研究所，大阪大学生物工学国际交流中心 |
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| 摘 要： | 报道了在生物反应器中供氧对紫苏细胞的影响。观察到在１００ｍＬ和５００ｍＬ三角摇瓶中进行液体培养时，改善供氧状况，有利于花色素的产生。在工作容量为２Ｌ的气泡反应器和３Ｌ的带螺旋桨的搅拌式反应器中，供氧的改善也使色素产量得到明显提高。但是，过高的通气量升所引直怕剪切力会对细胞产生破坏作用，反而不利于细胞生长和代谢。
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| 关 键 词： | 紫苏 供氧 花 色素 细胞培养 |
A Preliminary Test on the Effect of Oxygen Supplyon the Growth and Anthocyanin Production by Cell Cultures of Perilla frutescens in Bioreactors |
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| Zhong Jianjiang, Toshiomi Yoshida and Yu Juntang.A Preliminary Test on the Effect of Oxygen Supplyon the Growth and Anthocyanin Production by Cell Cultures of Perilla frutescens in Bioreactors[J].Journal of East China University of Science and Technology,1994,20(6):769-772. |
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| Authors: | Zhong Jianjiang Toshiomi Yoshida and Yu Juntang |
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| Abstract: | Effect of oxygen supply on the suspended cultures of Perilla fruteseens plantcells was demonstrated in various cultivation vessels.In flask cultures, Produetion of antho-cyanin pigment was increased with improvement of oxygen supply conditions.In cultivationsusing a 2L(working volume)bubble columm reactor and a 3L stirred reactor with a marineimpeller,the metabolite produetion was also conspieuously inereased by improving the condition of oxygen supply. However,it was observed that too strong aeration was harmful to thecell cultures,which may be due to high shear stress under the condition. |
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| Keywords: | anthocyanin bioreactor oxygen supply Perilla fruteseens plant cell culture |
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