| 肠激酶消化融合蛋白Trx-Exendin-4的特性 |
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| 引用本文： | 徐良,王文静,祖向阳,王小纯.肠激酶消化融合蛋白Trx-Exendin-4的特性[J].河南科学,2012,30(2):192-195. |
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| 作者姓名： | 徐良 王文静 祖向阳 王小纯 |
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| 作者单位： | 1. 河南农业大学生命科学学院,郑州,450002
2. 郑州牧业高等专科学校,郑州,450002 |
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| 基金项目： | 河南省科技攻关项目（0624420036） |
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| 摘 要： | 选用Invitrogen公司开发的重组肠激酶,在不同反应条件下消化融合蛋白Trx-Exendin-4,用16%的Tricine-SDS-PAGE检测目的多肽Exendin-4与其他切割产物的量.结果表明:温度对肠激酶消化融合蛋白Trx-Exendin-4的影响很大,37℃时酶活力高,以非特异性切割为主;16℃时酶活力居中,特异性切割与非特异性切割共存;4℃时酶活力较低,但以特异性切割为主.获得Exendin-4的最佳消化条件是:1 U肠激酶在4℃条件下6 h消化3 mg的Trx-Exendin-4,效率最高,特异性最好.
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| 关 键 词： | 肠激酶 Trx-Exendin-4 消化条件 非特异性切割 酶活力 |
Characteristics of Enterokinase Digesting on Recombinant Protein Trx-Exendin-4 |
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| Xu Liang
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Wang Wenjing
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Zu Xiangyang
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Wang Xiaochun.Characteristics of Enterokinase Digesting on Recombinant Protein Trx-Exendin-4[J].Henan Science,2012,30(2):192-195. |
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| Authors: | Xu Liang Wang Wenjing Zu Xiangyang Wang Xiaochun |
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| Institution: | 1. College of Life Science, Henan Agricultural University, Zhengzhou 450002, China; 2. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450002, China) |
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| Abstract: | The fusion protein Trx-Exendin-4 was digested by using the recombinant enterokinase of Invitrogen in different reaction conditions, then the peptide Exendin-4 and other product of nonspecific cutting were detected with 16% Tricine-SDS-PAGE. The result showed that temperature had large effects on the enterokinase digestion on recombinant protein Trx-Exendin-4. When digesting at 37 ℃, enterokinase had the highest activity without specificity; when digesting at 16 ℃, it had the moderate activity and specificity： when digesting at 4℃, it had the lowest activity but highest specificity. The optimal digestion condition for enterokinase was that one unit of enterokinase digested 3 mg of Trx-Exendin-4 at 4 ℃ for 6 h, which had the highest efficiency and specificity. |
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| Keywords: | enterokinase： Trx-Exendin-4： digestion condition： nonspecific cutting enzyme activity |
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