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| 荷花花瓣总RNA的提取方法 | |
| 引用本文： | 杨峰,李创,刘艺平,何明珍,孔德政.荷花花瓣总RNA的提取方法[J].河南科学,2009,27(10):1226-1228. |
| 作者姓名： | 杨峰 李创 刘艺平 何明珍 孔德政 |
| 作者单位： | 1. 河南农业大学,郑州,450002;青岛市政监理咨询有限公司,山东,青岛,266001 2. 河南农业大学,郑州,450002 3. 南阳市园林绿化管理局,河南,南阳,473000 |
| 摘 要： | 以荷花花瓣为材料,在比较CTAB-LiCl法和Trizol法的基础上,针对荷花花瓣富含多糖、多酚的特点,在RNA提取过程中加入去多糖步骤,改良了CTAB-LiCl法.结果表明,改良CTAB-LiCl法能有效地去除多糖,提取到的RNA 28 SrRNA亮度约为18SrRNA的二倍,A260/A280比值为1.98,A260/A230比值为2.36,RNA产率为685.3ng/μL,说明利用改良CTAB-LiCl法从荷花花瓣中提取的RNA质量好、产率高、完整性好. |
| 关 键 词： | 荷花 花瓣 RNA 改良CTAB-LiCl法 |
Extraction Methods of the Total RNA in the Petals of Lotus |
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| Yang Feng,Li Chuang,Liu Yiping,He Mingzhen,Kong Dezheng.Extraction Methods of the Total RNA in the Petals of Lotus[J].Henan Science,2009,27(10):1226-1228. | |
| Authors: | Yang Feng Li Chuang Liu Yiping He Mingzhen Kong Dezheng |
| Institution: | Yang Feng, Li Chuang, Liu Yiping, He Mingzhen, Kong Dezheng (1. Henan Agriculture University, Zhengzhou 450002, China： 2. Qingdao Municipal Supervision Consultative Co. Ltd, Qingdao 266001, Shandong China; 3. Nanyang Garden Administrative Bureau, Nanyang 473000, Henan China) |
| Abstract: | Two methods,CTAB-LiCl and Trizol,were used to extract total RNA from petals of lotus,and CTAB-LiCl was accordingly modified in extracting the total RNA in petals of lotus by integrating a polysaccharide-removing step in correspondence with the fact that lotus was rich in polysaccharides and polyphenol.The results showed that the modified CTAB-LiCl was capable of efficiently removing polysaccharides,and that of the RNAs extracted by the modified CTAB-LiCl,the lightness of 28 s rRNA was two times as much as that of 18 S Rrna, the value of A260/A280,A260/A230 was1.98,2.36,and the RNA yield reached 685.3 ng/μL,which explained that the modified CTAB-LiCl could extract high-quality and high-complete RNA at a higher productivity. |
| Keywords: | RNA |
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