| vvIBDV 诱导幼鸡免疫细胞凋亡的研究 |
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| 引用本文： | 张燕,赵晖,任前升,毛炳宇,王振光,张红卫.vvIBDV 诱导幼鸡免疫细胞凋亡的研究[J].山东大学学报(理学版),2003,38(2):105-107,111. |
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| 作者姓名： | 张燕 赵晖 任前升 毛炳宇 王振光 张红卫 |
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| 作者单位： | 山东大学,生命科学学院发育生物学研究所,山东,济南,250100 |
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| 基金项目： | 国家自然科学基金 (3 0 0 70 0 94)支持 |
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| 摘 要： | 为研究超强毒传染性法氏囊病病毒 (vvIBDV)感染的致病机理 ,在 3— 6周龄SPF幼鸡法氏囊、脾脏、胸腺细胞培养体系中加入vvIBDV ,以流式细胞术 (FcM)、DNA琼脂糖凝胶电泳技术检测vvIBDV诱导的细胞凋亡 ,结果表明 ,在本实验的体外培养体系中 ,vvIBDV可直接和迅速引起幼鸡法氏囊细胞凋亡 ,对脾脏或胸腺细胞并无明显的作用
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| 关 键 词： | vvIBDV 免疫细胞 细胞凋亡 细胞培养 |
| 文章编号： | 1671-9352(2003)02-0105-03 |
Apoptosis of Chicken Immunocytes Induced by vvIBDV |
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| Abstract: | To study the pathopoiesis of very virulent infectious bursal disease(vvIBDV),we studied the apoptosis of cultured bursa,spleen and thymus cells obtained from freshly isolated tissues of 3 6 week old SPF chickens cultured with vvIBDV. Apoptosis rate was analyzed by flow cytometry (FcM) and apoptosis was detected by agarose gel electrophoresis of extracted genomic DNA,Results showed that vvIBDV could induce apoptosis of 3 6 week old SPF chicken's bursa cells cultured in vitro,but had no apparent apoptosis inducing effect on spleen or thymus cells. |
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| Keywords: | vvIBDV immunocytes apoptosis cell culture |
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