| 连接臂对适体与凝血酶相互作用影响的电化学阻抗研究 |
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| 引用本文： | 彭亚鸽.连接臂对适体与凝血酶相互作用影响的电化学阻抗研究[J].延安大学学报(自然科学版),2012,31(3):64-68. |
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| 作者姓名： | 彭亚鸽 |
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| 作者单位： | 宁夏大学化学化工学院,宁夏银川,750021 |
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| 基金项目： | 国家自然科学基金青年基金资助项目(21005003) |
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| 摘 要： | 以5＇-C6-T0-A1,5＇-C6-T5-A1和5＇-C6-T10-A1三条不同长度连接臂修饰的凝血酶适体作为生物传感器的识别分子,结合巯基自组装技术,利用电化学阻抗技术,测定了凝血酶的线性范围和检出限,比较了三者灵敏度的差异。利用浓度平衡法计算了适体与凝血酶作用的结合常数,三条链的结合常数Ka分别为0.32 nM-1,0.84 nM-1和0.67 nM-1,表明,5＇-C6-T5-A1组装的传感器与凝血酶的结合常数最大,灵敏度最好。
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| 关 键 词： | 适体 连接臂 电化学阻抗 凝血酶 |
Effect of the Linker Structure to Aptamer-Thrombin Interaction Using Electrochemical Impedance Spectroscopy |
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| PENG Ya-ge.Effect of the Linker Structure to Aptamer-Thrombin Interaction Using Electrochemical Impedance Spectroscopy[J].Journal of Yan'an University(Natural Science Edition),2012,31(3):64-68. |
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| Authors: | PENG Ya-ge |
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| Institution: | PENG Ya-ge(College of Chemistry and Chemical Engineering,Ningxia University,Yinchuan 750021,China) |
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| Abstract: | The aptamer-thrombin interaction effect for the linker structure was studied using electrochemical impedance spectroscopy.Three linker structure aptamers,5’-C6-T0-A1,5’-C6-T5-A1 and 5’-C6-T10-A1,were immobilized on a gold electrode by the self-assembled technique.The linear range and the detection limit of the three aptasensors were obtained.The association constants Ka of the binding reaction of three aptamers with interaction of thrombin,were determined according to concentration equilibrium.The results showed that.association constants Ka is 0.32 nM-1,0.84 nM-1 and 0.67 nM-1respectively. |
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| Keywords: | aptamers linker structure electrochemical impedance spectroscopy thrombin |
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