两种消除Klebsiella pneumoniae重组型质粒的方法

对于工业发酵菌种肺炎克雷伯氏菌(Klebsiella pneumoniae),研究发现有两种消除其重组型质粒的有效方法,一种是连续传代培养,另一种是使用消除剂十二烷基硫酸钠(SDS)。对K.pneumoniae重组菌连续20代传代培养后,发现其质粒具有较高的消除率;而以0.2%SDS复合Ca2+处理K.pneumoniae重组菌,也能有效消除其重组型质粒,且该方法省却了反复的传代培养,能快速得到质粒消除菌,更具简便易操作性。消除了质粒的K.pneumoniae能再次接纳新的质粒,有效避免了因质粒不相容性带来的转化不成功,进而可用作宿主菌积累更多的生理性状。

Two methods for eliminating recombinant plasmids in Klebsiella pneumoniae

Good physiological performance of industrial microbes is crucial for successful bioprocessing. This generally requires the elimination of plasmids. In order to eliminate the recombinant plasmids in Klebsiella pneumoniae,two methods have been investigated,namely subculturing and sodium dodecyl sulphate (SDS) treatment. After 20 generations of subculturing,recombinant K. pneumoniae showed a high plasmid elimination ratio,and the recombinant strain treated by 0.2% SDS coupled with Ca2+ also showed a high plasm...