| 宏基因组技术在环境微生物资源开发中应用的探索 |
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| 引用本文： | 陆娟,任莉萍. 宏基因组技术在环境微生物资源开发中应用的探索[J]. 阜阳师范学院学报(自然科学版), 2005, 22(3): 35-37,42 |
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| 作者姓名： | 陆娟 任莉萍 |
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| 作者单位： | 阜阳师范学院,生物系,安徽,阜阳,236041 |
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| 摘 要： | 环境中约有99%的微生物在现有的条件下不能被培养,这使微生物资源的开发利用受到了限制.宏基因组是指某一特定的环境中全部微生物的总DNA,宏基因组技术是将样品中的总DNA提取出来后,利用适宜的载体克隆到宿主细胞中以构建成宏基因组文库,再筛选新的活性物质或基因,该技术在微生物资源的开发利用上将会有巨大的发展潜力.
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| 关 键 词： | 不可培养微生物 宏基因组 宏基因组技术 |
| 文章编号： | 1004-4329（2005）03-0035-03 |
| 收稿时间： | 2005-04-20 |
| 修稿时间： | 2005-04-20 |
A Primary Study of metagenome Technique in Development and Applicalion of Environmental Microbial Resource |
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| LU Juan,REN Li-Ping. A Primary Study of metagenome Technique in Development and Applicalion of Environmental Microbial Resource[J]. Journal of Fuyang Teachers College:Natural Science, 2005, 22(3): 35-37,42 |
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| Authors: | LU Juan REN Li-Ping |
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| Affiliation: | Department of Biology,Fuyang Teachers College, Anhui Fuyang 236041 ,China |
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| Abstract: | It is reported that in environment there is about 99% microorganisms which cannot be cultured under the present conditions by the traditional cultivation approach, which makes the development and utilization of microbial resource limited. 'Metagenome' is the genomes of the total microbe found in nature. Metagenome libraries are constructed by extracting DNA from environmental sample and transforming to surrogate host. By using the technique of metagenome there are enormous potentialities in microbial resource. |
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| Keywords: | non-culturable microbe metagenome technique of metagenome |
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