5 /6 肾切除小鼠中矿物质与骨代谢紊乱的特征

摘要: 目的通过制备5 /6 肾切除( 5 /6 Nx) 小鼠慢性肾脏病( CKD) 模型，观察小鼠成纤维生长因子23( FGF23) 及钙磷代谢指标，有助于研究矿物质与骨代谢紊乱的发病机制。方法28 只C57 雄性小鼠适应性饲养1 周后，切除模型组约5 /6 左肾，1周后切除右肾， 16 周后处死后收集血尿和肾脏组织标本，常规检测血尿FGF23、甲状旁腺素( PTH) 、活性维生素D、钙、磷等指标，观察肾脏病理。结果与假手术组和正常对照组相比，术后16 周5 /6 Nx 小鼠出现体质量减低，血清FGF23、PTH，血磷等异常升高，活性维生素D 水平显著降低，尿蛋白增加。肾脏病理发现系膜基质增生、胶原沉积及纤维化明显，和肾小球代偿性肥大。结论5 /6 Nx 小鼠可以出现明显的矿物质与骨代谢紊乱特征，是研究CKD 骨病合适的模型。

Chracteristics of Mineral and Bone Disorder in 5 /6 Nephrectomy Mouse Model

Abstract: Objective We produced the 5 /6 nephrectomy ( 5 /6 Nx) mice model of chronic kidney disease ( CKD) in order to observe FGF23，calcium phosphorus metabolism，and contribute to study the pathogenesis of CKDMBD．Method 28 C57 male mice were adapted for 1 week． Then the model group underwent unilateral renal ablation by 5 /6 Nx． Upper and low poles of the left kidney were cut． The right kidney was removed 1 week later．All the mice were executed at the 16th week． Blood，urine and kidney samples were collected． Then we detected FGF23，PTH，1，25( OH) 2D3，calcium，phosphorus and observed the renal histopathology． Result Comparedwith the sham group and the control group，the model group lost weight，had higher serum FGF23，PTH，phosphorus，urine output，urine protein and less 1， 25( OH) 2D3． Histopathology results found the kidney after 5 /6 Nx had proliferative mesangial matrix，increased collagen deposition，fibrosis and compensatory hypertrophy．Conclusion 5 /6 Nx mice had obvious characteristics of CKD-MBD，and is a good CKD-MBD model．