| 基于gyrB基因的地衣芽孢杆菌PCR快速检测方法的建立 |
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| 引用本文： | 李宏,吴海武,孟凡同,孙乐弟,谢珍玉,李立英.基于gyrB基因的地衣芽孢杆菌PCR快速检测方法的建立[J].海南大学学报(自然科学版),2013(3):230-233,239. |
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| 作者姓名： | 李宏 吴海武 孟凡同 孙乐弟 谢珍玉 李立英 |
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| 作者单位： | [1]海南大学热带生物资源可持续利用省部共建国家重点实验室培育基地,海南海口570228 [2]海南大学海南省水生生物技术重点实验室,海南大学海洋学院,海南海口570228 [3]广东轻工职业技术学院食品与生物工程系,广东广州510300 |
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| 基金项目： | 国家农业科技成果转化资金项目（2011GB2E200009）;国家科技支撑计划（2012BAC18B04）;海南省自然科学基金项目（311031） |
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| 摘 要： | 根据6株地衣芽孢杆菌(Bacillus licheniformis)的gyrB基因序列设计了1对特异性引物F1和R1,扩增产物大小为303 bp,以此引物对为基础,成功建立了灵敏度为1×10-3mg·L-1(约为200个细菌/反应)的地衣芽孢杆菌PCR快速检测方法 .该方法具有快速、灵敏度高、特异性强等优点,它为今后从微生物群落结构复杂的环境中分离地衣芽孢杆菌奠定了良好的基础.
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| 关 键 词： | 地衣芽孢杆菌 gyrB基因 PCR |
Development of A PCR Method for Rapid Detection of Bacillus licheniformis Based on gyrB Gene |
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| LI Hong;WU Hai-wu;MENG Fan-tong;SUN Le-di;XIE Zhen-yu;LI Li-ying.Development of A PCR Method for Rapid Detection of Bacillus licheniformis Based on gyrB Gene[J].Natural Science Journal of Hainan University,2013(3):230-233,239. |
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| Authors: | LI Hong;WU Hai-wu;MENG Fan-tong;SUN Le-di;XIE Zhen-yu;LI Li-ying |
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| Institution: | LI Hong;WU Hai-wu;MENG Fan-tong;SUN Le-di;XIE Zhen-yu;LI Li-ying(State Key Laboratory Breeding Base for Sustainable Exploitation of Tropical Biotic Resources, Hainan University, Haikou 570228, China;Key Laboratory of Aquatic Biological Technology of Hainan, Ocean College , Hainan University, Haikou 570228, China;Department of Food and Biological Engineering, Guangdong Industry Technology College, Guangzhou 510300, China) |
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| Abstract: | In this study,based on gyrB gene sequences from six Bacillus licheniformis isolates,a pair of specific primers,F1 and R1,was designed.The primers were used for the detection of Bacillus licheniformis,the size of the amplified product and the sensitivity were 303 bp and 1 × 10-3 mg · L-1,respectively.The method had the advantages of high-efficiency,high-sensitivity and high-specificity,and which set a good foundation for isolating Bacillus licheniformis from a complex microbial community samples. |
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| Keywords: | Bacillus licheniformis gyrB gene PCR |
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