原位PCR技术在植物学中的应用

随着聚合酶链式反应(Polymerase chain reaction,PCR)技术和分子水平生物技术的发展,综合了原位杂交(Insitu Hybridize,ISH)和PCR双重优点的原位PCR(Insitu polymerase chain reaction,IS-PCR)在越来越多的领域得到应用,或者具有巨大的应用前景。目前关于IS-PCR的报道主要集中在动物学和微生物学等领域,植物学领域较少。现从植物外源基因的检测、基因定位、高分辨率的DNA物理图谱的构建和植物种属关系的鉴定等方面就IS-PCR技术在植物学中的应用进行了综述。

The application of In Situ PCR technology in plant science

With development of polymerase chain reaction (PCR) technology and other progress of biotechnology at molecular level,the In situ polymerase chain reaction (IS-PCR) has increased applications and prospects in many fields because of its integrated advantages in both PCR and In situ hybridization (ISH). At present,reports about the application of IS-PCR mainly focused on zoology and microbiology. This paper has reviewed the application of IS-PCR on plant sciences such as gene test,gene location,construction of high-resolution physical map of DNA and inter-specific phylogeny.