| Nafion膜固定血红蛋白和银纳米粒子构筑NO_2~-生物传感器 |
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| 引用本文： | 李艳彩,莫韬.Nafion膜固定血红蛋白和银纳米粒子构筑NO_2~-生物传感器[J].五邑大学学报(自然科学版),2010,24(3):14-19. |
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| 作者姓名： | 李艳彩 莫韬 |
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| 作者单位： | 漳州师范学院,化学与环境科学系,福建,漳州,363000 |
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| 基金项目： | 福建省教育厅科技项目，漳州师范学院科研基金资助项目 |
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| 摘 要： | 利用Nafion膜将血红蛋白(Hb)和银纳米粒子(AgNPs)固载到玻碳电极表面制作N O-2生物传感器.循环伏安实验表明:Hb/AgNPs/Nafion膜电极在pH值为6.9的PBS缓冲溶液中呈现出一对明显的HbFe(Ⅲ)/(Ⅱ)氧化还原峰,该电极对N O2-有良好的催化作用,线性范围为2.0×10-5~3.4×10-4 mol/L(n=18,R=0.997),检测限为1.2×1 0-6 mol/L(信噪比为3);故该膜电极可做N O-2生物传感器.
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| 关 键 词： | 血红蛋白 Nafion 银纳米粒子 NO2^-生物传感器 |
Building Biosensors by Immobilizing Hemoglobin and Silver Nanoparticles and Using Nafion Membrane |
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| LI Yan-cai,MO Tao.Building Biosensors by Immobilizing Hemoglobin and Silver Nanoparticles and Using Nafion Membrane[J].Journal of Wuyi University(Natural Science Edition),2010,24(3):14-19. |
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| Authors: | LI Yan-cai MO Tao |
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| Institution: | (Chemistry and Environmental Science Department,Zhangzhou Teachers College,Zhangzhou 363000,China) |
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| Abstract: | Hemoglobin（Hb） and silver nanoparticles（AgNPs） are immobilized to the surface of glassy carbon electrode by using Nafion membrane.Cyclic voltammetry experiments show that Hb/AgNPs/ Nafion film electrode in pH 6.9 PBS buffer solution has a pair of significant HbFe（Ⅲ）/（Ⅱ） oxide reduction peak and has a good catalytic response to of nitrite.The linear range is 2.0×10-5-3.4×10-4 mol/L（n=18,R=0.997） and the detection limit is 1.2×10-6 mol/L（SNR=3）.Therefore,the membrane electrode can be used as nitrite biosensor. |
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| Keywords: | Nafion |
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