SSR标记对籼稻品种的遗传多样性分析

利用500多对SSR标记对广陆矮4号、珍汕97B、佳辐占、明恢86和明恢63 5个籼稻亲本品种进行遗传多样性分析.结果表明,佳辐占与明恢63、明恢86、珍汕97B和广陆矮4号之间的相似系数分别为78.1%、76.1%、72.8%和71.7%.相对而言,其相似系数越小,遗传距离越大,因此利用佳辐占与这些品种组配较有希望选育出品质优良、综合优势明显的新品系.同时研究表明,标记间平均距离为10 cM或小于10 cM时,分析所得结果较为可靠.

Genetic Diversity Analysis Based on SSR in Indica Varieties

Genetic diversity between rice parents is the key role for rice breeding. Using of hybrid advantage is important to improve the output and quality of rice. Fully understanding of the genetic background of rice parents has significant meaning in guidance of choosing hybrid rice parent and exploring new genetic resource in rice. In this paper,we detected and analyzed genetic diversity between Jiafuzhan and other four candidate indica varieties by using more than 500 pairs of SSR markers. It shows that the similarity coefficient between Jiafuzhan and Minghui63, Minghui86,Zhenshan97B, and Guangluai4 is 78.1%, 76. 1%, 72.8% and 71.7%, respectively. The similarity coefficients between Jiafuzhan and Minghui series are higher than Jiafuzhan and the rest two candidates. In general,the similarity coefficients among these 5 varieties are relatively lower, and genetic distances are relatively higher. There is high possibility to cultivate a new elite variety with distinct comprehensive superiority by hybriding Jiafuzhan with the rest varieties. At the same time,we discuss the most appropriate number of markers in genetic diversity analysis. It shows that the result of genetic diversity analysis is more reliable when the SSR interval on the chromosomes is about 10 cM or fewer.