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| 酵母提取物对水母雪莲悬浮培养合成紫丁香苷的影响 | |
| 引用本文： | 徐春明,赵兵,欧元. 酵母提取物对水母雪莲悬浮培养合成紫丁香苷的影响[J]. 北京工商大学学报(自然科学版), 2009, 27(3) |
| 作者姓名： | 徐春明 赵兵 欧元 |
| 作者单位： | 1. 北京工商大学,北京市植物资源研究开发重点实验室,北京,100048 2. 中国科学院过程工程研究所,生化工程国家重点实验室,北京,100080 3. 公安部物证鉴定中心,北京,100038 |
| 摘 要： | 研究了酵母提取物诱导的水母雪莲悬浮培养时紫丁香苷生产和相关次级代谢酶的活性.酵母提取物促进了紫丁香苷的生产,最优的添加方法是在培养15 d时添加1.5%的酵母提取物.最大紫丁香苷产量达671.9 mg/L,是对照实验的3.25倍.诱导子处理后G6DPH、PAL和POD活性显著增加,当处理6 h后酶活性达到最大值. |
| 关 键 词： | 诱导子 水母雪莲 紫丁香苷 悬浮培养 |
EFFECT OF YEAST EXTRACT ON SYRINGIN PRODUCTION IN SUSPENSION CULTURES OF SAUSSUREA MEDUSA |
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| XU Chun-ming,ZHAO Bing,OU Yuan. EFFECT OF YEAST EXTRACT ON SYRINGIN PRODUCTION IN SUSPENSION CULTURES OF SAUSSUREA MEDUSA[J]. Journal of Beijing Technology and Business University:Natural Science Edition, 2009, 27(3) | |
| Authors: | XU Chun-ming ZHAO Bing OU Yuan |
| Affiliation: | 1.Beijing Key Laboratory of Plant Resources Research and Development;Beijing Technology and Business University;Beijing 100048;China;2.State Key Laboratory of Biochemical Engineering;Institute of Process Engineering;Chinese Academy of Sciences;Beijing 100080;3.Institute of Forensic Science Ministry of Public Security;Beijing 100038;China |
| Abstract: | Syringin production and related secondary metabolism enzyme activities in suspension cultures of saussurea medusa treated with yeast extract were investigated.Yeast extract enhanced syringin production,and the optimal feeding protocol was the addition of 1.5%(v/v) yeast extract at the 15th day of the cell culture.The highest syringin production reached 671.9 mg/L,which was 3.25-fold that of the control.The glucose-6-phosphate dehydrogenase(EC 1.1.1.49),phenylalanine ammonia lyase(EC 4.3.1.5) and peroxidase(... |
| Keywords: | elicitor saussurea medusa syringin suspension cultures |
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