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臭氧对草鱼鱼种血液MDA GSH 浓度以及GPX 活性的影响
引用本文:徐军, 马广智. 臭氧对草鱼鱼种血液MDA GSH 浓度以及GPX 活性的影响[J]. 华南师范大学学报(自然科学版), 2004, (2): 110.
作者姓名:徐军  马广智
作者单位:华南师范大学生命科学学院,广东,广州,510631
摘    要:在实验室条件下,用质量浓度为0.118和0.278mg/L的臭氧处理草鱼鱼种,研究臭氧对草鱼血液中丙二醛(MDA)、谷胱甘肽(GSH)浓度和谷胱甘肽过氧化物酶(GPX)活性的影响.实验结果表明:MDA浓度在两种质量浓度中暴露30min后显著升高.GSH浓度在0.118mg/L暴露90min后显著降低,在0.278mg/L暴露30min后显著降低.GPX活性在0.118mg/L暴露30min显著升高,并在90min的实验时间内维持在一恒定水平;在O.278mg/L暴露30min其活性显著升高,但之后便呈现下降趋势,并在第90min时显著低于对照组.

关 键 词:臭氧  草鱼  丙二醛  谷胱甘肽过氧化物酶  GPX  活性  水产养殖  毒性
文章编号:1000-5463(2004)02-0110-04
修稿时间:2003-09-02

EFFECTS OF OZONE ON GPX ACTIVITY AND CONTENT OF GSH AND MDA IN BLOOD OF GRASS CARP (Ctenopharygodon idellus) FINGERLING
XU Jun,MA Guang-zhi. EFFECTS OF OZONE ON GPX ACTIVITY AND CONTENT OF GSH AND MDA IN BLOOD OF GRASS CARP (Ctenopharygodon idellus) FINGERLING[J]. Journal of South China Normal University(Natural Science Edition), 2004, 0(2): 110-113
Authors:XU Jun  MA Guang-zhi
Abstract:The effects of ozone on the activity of GPX and the content of GSH and MDA were studied by using the blood of 1-year-old grass carp (Ctenopharygodon idellus) fingerling exposed to the ozone concentration of 0.118 mg/L and 0.278 mg/L in fresh water for 30 min, 60 min and 90 min respectively. At the concentration of 0.118 mg/L and 0.278 mg/L, the content of MDA was significantly elevated after 30 min. The content of GSH in blood was significantly decreased after 90 min exposure of 0.118 mg/L ozone and after 30min of 0.278 mg/L. The activity of GPX was elevated significantly at the concentration of 0.118 mg/L after 30min and then kept at a invariable level until 90 min, but at 0.278 mg/L, it was elevated significantly after 30 min and then decreased which is significantly after 90 min.
Keywords:Ozone  grass carp (Ctenopharygodon idellus)  MDA  GSH  GPX
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