| 鸡肝二氢叶酸还原酶的亲和层析提取及其抑制剂抑制活性的测定 |
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| 引用本文： | 张馨月,栗娜,毛亚,朱若华. 鸡肝二氢叶酸还原酶的亲和层析提取及其抑制剂抑制活性的测定[J]. 首都师范大学学报(自然科学版), 2008, 29(5) |
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| 作者姓名： | 张馨月 栗娜 毛亚 朱若华 |
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| 作者单位： | 首都师范大学化学系,北京,100037 |
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| 摘 要： | 利用氨甲喋呤-氨基己基-琼脂糖凝胶(Methotrexate-Aminohexyl-Sephamse,MTX-AH-Sepharose)亲和层析法,从鸡肝中得到了具有一定纯度(比活力为22.67 units/mg)的二氢叶酸还原酶(Dihydrofolate reductase,DHFR),通过紫外光度法测定了氨甲喋呤(Methotrexate,MTX)和表没食子儿茶素没食子酸酯((-)-Epigallocatechin gallate,EGCG)对纯化所得的DHFR的抑制效果.
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| 关 键 词： | 二氢叶酸还原酶 抑制剂 亲和层析 鸡肝 |
Purification of Dihydrofolate Reductase from Chicken Liver by Affinity Chromatography and Determination of Inhibition Activity of its Inhibitors |
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| Zhang Xinyue,Li Na,Mao Ya,Zhu Ruohua. Purification of Dihydrofolate Reductase from Chicken Liver by Affinity Chromatography and Determination of Inhibition Activity of its Inhibitors[J]. Journal of Capital Normal University(Natural Science Edition), 2008, 29(5) |
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| Authors: | Zhang Xinyue Li Na Mao Ya Zhu Ruohua |
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