| 碱性蛋白酶交联酶晶体的制备及稳定性 |
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| 引用本文： | 潘力 林炜铁. 碱性蛋白酶交联酶晶体的制备及稳定性[J]. 华南理工大学学报(自然科学版), 1998, 26(9): 22-26 |
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| 作者姓名： | 潘力 林炜铁 |
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| 作者单位： | 华南理工大学食品与生物工程学院 |
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| 摘 要： | 研究了用透析平衡法制备碱性蛋白酶晶体的过程，在硫酸铵饱和度为２５％的条件下获得了符合要求的碱性蛋白酶晶体，然后采用戊二醛进行交联，制备出了交联酶晶体，并对其在高酸碱度、高温、有机溶剂或有机溶液中的稳定性进行了研究．实验结果表明，交联酶晶体有较高的稳定性．
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| 关 键 词： | 碱性蛋白酶；交联酶晶体；稳定性 |
THE PREPARATION AND STABILITY OF ALKALINE PROTEASE IN FORM OF CROSS_LINKED ENZYME CRYSTALS |
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| Pan Li Lin Weitie Yao Ruhua. THE PREPARATION AND STABILITY OF ALKALINE PROTEASE IN FORM OF CROSS_LINKED ENZYME CRYSTALS[J]. Journal of South China University of Technology(Natural Science Edition), 1998, 26(9): 22-26 |
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| Authors: | Pan Li Lin Weitie Yao Ruhua |
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| Abstract: | This paper studies the preparation of crystals of alkaline protease by dialysis equilibrium. Crystals of alkaline protease are grown from 25% saturated ammonium sulfate and cross_linked with glutaraldehyde to produce cross_linked enzyme crystals. Also dealt with is its stability in different environments, including high pH extremes and temperature, with the exposure to organic solvents or aqueous_organic mixtures. The result demonstrates that the stability of alkaline enzyme in form of CLECs is high. |
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| Keywords: | alkaline protease cross_linked enzyme crystals stability |
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