| 荧光光谱法研究茜素红S与壳聚糖的结合反应 |
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| 引用本文： | 高贵珍,刘小阳.荧光光谱法研究茜素红S与壳聚糖的结合反应[J].安徽大学学报(自然科学版),2010,34(4). |
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| 作者姓名： | 高贵珍 刘小阳 |
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| 作者单位： | 宿州学院,生化分析研究室,安徽,宿州,234000 |
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| 基金项目： | 安徽省教育厅自然科学基金资助项目 |
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| 摘 要： | 研究生物探针茜素红S和壳聚糖溶液的荧光光谱特征,用荧光光度法、分光光度法研究茜素红S与壳聚糖分子之间的结合反应,结果表明:其能量转移机制为非辐射能量转移,论证了茜素红S与壳聚糖结合作用为静态猝灭过程(Kq=2.958 1×1011L.mol-1.s-1).测定了其结合常数(KA=3.26×103)和结合位点数(n=102).
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| 关 键 词： | 荧光光谱 茜素红S 壳聚糖 |
Study on the interaction between alizarin red S and chitosan by fluorescence method |
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| GAO Gui-zhen,LIU Xiao-yang.Study on the interaction between alizarin red S and chitosan by fluorescence method[J].Journal of Anhui University(Natural Sciences),2010,34(4). |
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| Authors: | GAO Gui-zhen LIU Xiao-yang |
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| Institution: | GAO Gui-zhen,LIU Xiao-yang(Biochemistry Analysis Laboratory,Suzhou College,Suzhou 234000,China) |
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| Abstract: | The binding interaction between alizarin red S(ARS) and chitosan(CTS) has been studied by fluorescence and absorption spectra.The results showed that the quenching mechanism was a static quenching procedure(Kq=2.958 1×1011 L·mol-1·s-1) and the energy transfer mechanism was a nonradiative procedure.The equilibrium constant(KA=3.26×103) and number of binding sites(n=102) were obtained. |
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| Keywords: | fluorescence spectra alizarin red S chitosan |
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