不同配方高脂饲料构建高血脂症大鼠模型的比较及评价

摘要：目的 在大鼠高脂饲料中添加膨润土以提高高脂饲料的硬度及可食性，探讨其与传统高脂饲料配方相比，对建立高血脂大鼠模型的作用。方法 雄性SD大鼠50只，按体重随机分成对照组10只、普通高脂饲料组和膨润土高脂饲料组各20只。动物自由摄食饮水，每周称量大鼠体重，实验前后检测大鼠血清中的总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）的含量,喂养8W后测量每只大鼠的体长、脂肪重量（肾周脂肪和附睾脂肪），计算其脂体比和Lee’s 指数。结果 1、膨润土高脂饲料组体重显著高于对照组（P＜0.01），与高脂饲料组相比增高不明显。2、与对照组比较，高脂饲料组和膨润土高脂饲料组血清TC、TG和LDL-C的水平均显著增加（P＜0.01）。高脂饲料组和膨润土高脂饲料组血清中HDL-C有降低的趋势，膨润土高脂饲料组降低的趋势更明显，但差异均无统计学意义。与高脂饲料组比较，膨润土高脂饲料组TC水平显著增高（P＜0.05）。3、与对照组相比，高脂饲料组和膨润土高脂饲料组大鼠身长、肾周脂肪重量、附睾脂肪重量、脂体比和Lee’s指数显著增加（P＜0.05）。同时与高脂饲料组相比，膨润土高脂饲料组附睾脂肪重量显著增加（P＜0.05）。结论 高脂饲料组和膨润土高脂饲料组饲料喂养SD大鼠8周后，均能够诱导大鼠高脂血症模型，但膨润土高脂饲料组诱导高脂血症大鼠模型优于普通高脂饲料组。

Comparison and Evaluation of Rats Model with Hyperlipidemia Fed with Different Formula of High- fat Diet

Abstract: Objective Add bentonite to the rats' high-fat diet to improve its hardness and edibility,and the effect on the establishment of hyperlipidemic rat model was compared with traditional high-fat diet. Method Fifty male SD rats were randomly divided into control group (n = 10), traditional high-fat diet group and bentonite-added group (n = 20, respectively).The animals were allowed to eat and drink freely, and the rats were weighed weekly, the levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) were measured before and after the experiment. After 8 weeks' feeding, each rat's body length and weight of fat (perirenal fat and epididymal fat) were measured, and the fat ratio and Lee's index were calculated. Results 1).Weight of the rats in bentonite-added group increased significantly compared to the control group (P <0.01), which increased not significantly compared to the traditional high-fat diet group. 2).The levels of TC,TG and LDL-C in serum in traditional high-fat diet group and bentonite-added group were all increased significantly compared to the control group(P <0.01). The level of HDL-C in serum in both traditional high-fat diet group and bentonite-added group were showed a trend to decrease, which was more obvious in the bentonite-added group, but the difference was not statistically significant. The level of TC in the bentonite-added group increased significantly compared to the traditional high-fat diet group (P <0.05). 3).The rats' body length, the weight of perirenal fat, the weight of epididymal fat, fat ratio and Lee's index in both traditional high-fat diet group and bentonite-added group were increased significantly compared to the control group(P <0.05). Meanwhile, the weight of epididymal fat increased significantly in bentonite-added group compared to the traditional high-fat diet group (P <0.05). Conclusion Both traditional high-fat diet and bentonite-added diet could lead to the rats' hyperlipidemia after 8 weeks' feeding, the models induced by the bentonite-added diet was better than those induced by the traditional high-fat diet.