补肾活血颗粒对多囊卵巢综合征大鼠血清性激素水平、 血糖及胰岛素的影响

目的 观察补肾活血颗粒对多囊卵巢综合征（PCOS）模型大鼠血清性激素水平、血糖及胰岛素的影响。方法 采用颈背部皮下注射脱氢表雄酮（DHEA）造模法建立PCOS大鼠模型，将72只雌性SD大鼠随机分为正常对照组、模型组、二甲双胍组、中药高剂量组、中药中剂量组、中药低剂量组，每组12只，各组给予相应药物连续灌胃 20 d，用酶联免疫吸附测定法测定血清17-羟孕酮（17-OHP）、促黄体生成素（LH）、睾酮（T）、促卵泡素（FSH）、空腹 血糖（FPG）及胰岛素（INS）水平。结果模型组血清中17-OHP、LH、T、FSH、FPG、INS水平明显高于正常对照组 (P<0.01 ，P<0. 05 )，中药高剂量组降低大鼠血清LH、T、FSH及INS水平最为明显(P<0.01),二甲双胍组、中药中剂量组显著降低FPG（P<0. 01）,中药各治疗组均能降低17-OHP（P<0. 05）。结论 补肾活血颗粒能对PCOS 大鼠有一定的治疗作用，可改善激素水平、血糖及胰岛素的异常

Effects of BuShen HuoXue Particle on Sex Hormone Levels,Blood Sugar and Insulin of PCOS Rats

Abstract: Objective To observe the effects of BuShen HuoXue Particle on sex hormone levels , blood sugar and insulin of PCOS rats. Method We established the model of PCOS rats using subcutaneous injection of DHEA mode method .Seventy-two Rats were randomly divided into 5 groups: normal control group , model control group , Metformin group, bushen HuoXue of high dose group , middle dose group and 10W dose group of herbs , 12 rats each group. Rats in all groups were treated with corresponding drugs by gastrogavage for 20 days. Determination of 17 - hydroxy serum progesterone ( 17 - OHP) , detected luteinizing hormone ( LH ) , testosterone, follicle stimulating hormone ( FSH ) , fasting plasma glucose( FPG ) and insulin ( INS ) were detected by adopting ELISA method . Result Model group serum 17 -OHP , FSH , LH , T , FPG , INS levels are significantly higher than the normal control group ( P<0. 01 , P<0. 05 ) , Traditional Chinese medicine ( TCM ) in high dose group of lower rat serum LH , T, FSH and INS level is most obvious ( P<0. 01 ) , Metformin group and the Chinese medicine dose group significantly lower FPG ( P t0.01 ) , Chinese medicine in each treatment group can reduce17 -OHP ( P<0.05 ) . Conclusion BuShen HuoXue Particle could effectively improve endocrine levels of PCOS rats, improve the abnormal level, blood glucose and insulin.