颅脑损伤后认知障碍大鼠模型的制备及评价

目的 探索一种颅脑损伤后认知障碍动物模型的制备及评价。方法 健康雄性大鼠(Sprague Dawley，SD)60只，水迷宫筛取48只纳入实验，随机分空白对照、模型对照和模型组。模型制备采用电子颅脑损伤仪(electric Cortical Contusion Impactor,eCCI)打击，配合水迷宫筛选确定颅脑损伤(Traumatic brain injury，TBI)后认识障碍大鼠。观测大鼠生命体征、神经功能及运动功能；Morris水迷宫实验；测血清中MMP-2、MMP-9、SOD、MDA含量变化。结果 与空白组比较：模型组生命体征稳定、各行为学指标均存在差异(P<0.05)，水迷宫实验潜伏期和空间探索实验结果均存在明显差异(P<0.01)，血清中MMP-2、MMP-9、MDA含量明显上升(P<0.01)，SOD明显下降(P<0.01)。结论 本模型制备策略能够较好模拟颅脑损伤后的认知障碍，同时稳定性、重复性好、评价客观，又易操作掌握，可供同类研究时借鉴参考。

Establishment and Evaluation of a Rat Model of Cognitive Impairment after Traumatic Brain Injury

Objective To explore the preparation and evaluation of an animal model of cognitive impairment after Traumatic brain injury.Method Sixty healthy male SD rats and the remaining 48 rats after water maze screening were randomly divided into blank control group,model control group and model group.The electric Cortical Contusion Impactor was used to attack the model and water maze screening was used to determine the cognitive impairment rats after TBI.The changes of vital signs,nerve function and motor function,Morris water maze test,contents of MMP-2,MMP-9,SOD and MDA were observed.Result Compared with the blank group,there were significant differences in vital signs and behavioral indicators (P<0.05),latency of water maze test and space exploration test (P<0.01),serum MMP-2,MMP-9 and MDA levels (P<0.01),and SOD levels (P<0.01).Conclusion The model preparation scheme proposed in this paper can simulate the cognitive impairment after craniocerebral injury,and has good stability,reproducibility,Objective evaluation,and better operation of animal model platform and evaluation system,which is worthy of reference in similar studies.