巴马猪椎间盘退变模型的建立

目的 建立巴马猪腰椎间盘退变模型。方法 巴马小型猪6头，每组3头，分别为对照组(Con组)，造模组(Mod组)。Con组不进行任何处理， Mod组在C形臂X光引导下确定猪L2-3、L3-4、L4-5、L5-6椎间隙，用1%的利多卡因进行浸润麻醉，用16G的引导针沿定位点穿刺椎间盘。穿透纤维环后侧后，呈放散状将椎间盘进行针刺破坏，每个间盘进行6次穿刺，每个角度间隔30度，分别于实验前及实验后1个月对巴马猪腰椎间盘行放射学及组织学观察。结果 Con组椎间盘未见明显变化， Mod组行MRI检查，发现进行穿刺的椎间盘出现间隙变窄，椎间盘后突，硬膜囊受压，髓核信号缺失，纤维环变形等改变，按照Pfirrmann分级标准，处理后的椎间盘在Ⅲ～Ⅳ级；病理组织学观察，与Con组比较，Mod组的椎间盘髓核呈白色胶冻状，穿刺部位椎间盘厚度下降，髓核发暗、含量减少，通道周围组织广泛硬化，纤维环结构粗糙。结论 C形臂X光定位下通过针刺破坏巴马小猪纤维环及椎间盘的方法，可以获得一种可靠的椎间盘退行病变模型。

Establishment of Lumbar Degenerative Animal Experimental Model in Bama miniature pigs

Abstract: Objective To establish lumbar degenerative animal experimental model in Bama miniature pigs. Method Six Bama miniature pigs were divided into the control group (Con group) and model group (Mod), which contained 3 pigs, respectively. Con group was not treated, in mod group after infiltrating anesthesia with 1% lidocaine, 16G guided needle was inserted into L2-3, L3-4, L4-5 and L5-6 intervertebral disc to damage the tissue, 6 times of puncture for each intervertebral disc. After 1 month, radiological and histological observation of Bama miniature pigs lumbar intervertebral disc performed. Result Con group of intervertebral disc saw no obvious changes, MRI of the Mod group showed narrow and clearance in intervertebral disc. And dural sac was compressed, nucleus pulpous signal was missed, and fiber ring deformation change was observed. According to the Pfirrmann grading standard, Ⅲ—Ⅳ level alteration of intervertebral disc was found in Mod group. Compared with Con group, histological change in the Mod group showed a white disc nucleus with pulpous gelatinous appearance, decreased disc thickness, content reducing in pith nucleus, dark tissue and fibrosis channel-surrounding area. Conclusion Under the DSA positioning, through the fiber ring and disc puncture in Bama miniature pigs, a reliable animal model of intervertebral disc degeneration disease was established.