用五种标准物质的稳定性评价实验动物遗传质量研究

目的 通过分析5种标准物质的稳定性，获得标准物质的贮存条件和运输条件。方法 依据国家标准 GB/T 14927.1—2008进行标准物质的制备，设定-20℃，4℃，室温和37℃为4个温度检验点，设定1、2、3、7、14、30、60 d和90 d为8个时间检验点，进行稳定性检验。结果 5种标准物质在4 ℃和-20 ℃均可以稳定保存至90 d。过氧化氢酶-2在37 ℃能稳定保存3 d，其他4种标准物质在37 ℃仅能稳定保存1 d。在室温条件下，苹果酸酶-1，异柠檬酸脱氢酶-1和过氧化氢酶-2能稳定保存3 d，碱性磷酸酶-1能稳定保存5 d，肽酶-3能稳定保存14 d。结论 用于实验动物遗传质量评价使用的标准物质，有必要严格控制其保存条件和运输条件。

Study on the Stability of 5 Standard Substances for the Evaluation of Genetic Quality in Laboratory Animals

Objective To investigate the stability of 5 standard substances in order to obtain the storage condition and transportation condition of standard substance. Method On the basis of the national standard GB/T14927.1—2008, standard substances were prepared, stability test were carried on 4 temperatures(-20 ℃，4℃，room temperature and 37 ℃) and 8 time points (1 day、2 days、3 days、7 days、14 days、30 days、60 days and 90 days). Result 5 standard substances can be stably preserved during 90 days at 4 ℃ and -20 ℃. Catalase 2 standard substance can be preserved during 3 days at 37 ℃, the other 4 standard substances can be preserved only in 1 day at 37 ℃. At room temperature, malic enzyme 1, isocitric dehydrogenase 1 and catalase 2 can be preserved during 3 days. Alkaline phosphatase 1 can be preserved during 5 days. Peptidase 3 can be preserved in 14 days. Conclusion The preservation and transportation conditions of the standard substances must be controlled strictly during genetic quality evaluation of laboratory animals.