MuSK重症肌无力小鼠模型的建立

目的 建立肌肉特异性受体酪氨酸激酶抗体(MuSK-Ab)阳性的重症肌无力小鼠模型。方法 6～8周龄的雌性C57BL/6小鼠(40只)，适应饲养1周后，随机分组后造模。造模第1天在 4个位置(两个后脚和两个肩膀)分别注射免疫抗原(每个部位50 μL，含12.5μg 鼠源肽，CFA完全费氏佐剂)；造模第30及50天再次在上述 4个位置(两只后脚和两个肩部)分别注射免疫抗原，第70天后每天对发病情况进行评分。结果 模型成功率50%。评分为1分小鼠1只，2分的3只，3分的5只，4分为1只。结论 制备MuSK-Ab小鼠模型,造模方法可行,有助于对重症肌无力疾病的深入研究。

Establishment of a Standardized MuSK Myasthenia Gravis Mouse Model

Objective To Model a muscle-specific receptor tyrosine kinase antibody (MuSK-Ab)-positive myasthenia gravis mouse model according to international standardization tablets.Method Female C57BL/6 mice (40 mice)aged 6-8 weeks were conditioned for 1 week and randomly modeled.On the first day of modeling,immunization antigens were injected at four locations (two hind legs and two shoulders)(50 μL per site,containing 12.5μg of mouse peptide,CFA complete Freund’s adjuvant);modeling days 30 and 50 Immune antigens were injected at four locations (two hind legs and two shoulders)and the onset was scored daily after day 70.Result The model success rate is 50%.The score is 1 point for 1 mouse,2 points for 3,3 points for 5,4 for 1.Conclusion The preparation of the MuSK-Ab mouse model according to international standardization is helpful for in-depth study of myasthenia gravis.