SLC1A2基因SNP位点与拉布拉多和金毛猎犬活跃度的相关性研究

目的 检测SLC1A2基因SNP位点与拉布拉多和金毛猎犬活跃度的相关性，以期为导盲犬早期选育筛选有效的遗传学分子标记。方法 选取中国大连导盲犬培训基地的99只犬(拉布拉多犬80只，金毛猎犬19只)，采用Passive test(PT)行为测试法对犬的活跃度进行评估。同时采集该99只犬的静脉血，提取血细胞中的基因组DNA样本，采用PCR/测序方法对该99只犬的SLC1A2基因T129C、T1549G 和T471C 3个SNP位点进行基因型鉴定，并统计分析犬的活跃度与SLC1A2 SNP位点的相关性。结果 SLC1A2 基因的3个SNP位点中，T129C和T1549G位点没有多态性，分别仅存在C/C型和G/G型。T471C位点存在3种基因型：T/T、T/C和C/C。这3种基因型与活跃度、性别以及品种间不存在显著相关性(P>0.05)。另外，品种与活跃度之间也没有显著相关性(P>0.05)，但性别与活跃度间存在显著相关性(P=0.049)。结论 SLC1A2基因的T129C、T1549G 和T471C位点与导盲犬的活跃度不具有显著相关性，不能作为导盲犬的遗传学筛选的指标。由于性别与活跃度间存在显著相关性，因而在行为学测试中，对犬的活跃度评分标准应雌雄有别。

Correlation between SNP Loci of SLC1A2 and the Activity Level of Labrador and Golden Retriever

Objective To determine the correlation between the SNP loci of SLC1A2 and the activity level of Labrador and Golden Retriever for selection of effective genetic biomarkers.Method Ninety-nine dogs (80 Labrador and 19 Golden)were selected from the Chinese guide dog training center of Dalian.The activity levels of the dogs were evaluated by Passive Test.Venous bloods of the dogs was collected and the genomic DNA was isolated from white blood cells.Three SNP loci of SLC1A2 gene,T129C,T1549G,and T471C,were detected by PCR/sequencing method .Finally,the correlations between activity level and genotypes were analyzed.Result The T129C and T1549G loci of SLC1A2 gene were not polymorphic.The T471C locus had three genotypes:T/T,T/C and C/C.There was no significant correlation between the three genotypes of T471C and the activity level,sex,and breed (P>0.05).There was also no significant correlation between the activity level and the breed.However,there was significant correlation between activity level and sex (P=0.049).Conclusion There is no significant correlation between SNP loci of SLC1A2 gene and activity levels of Labrador and Golden Retriever.