西方膳食对载脂蛋白E敲除小鼠的影响

目的 研究西方膳食和标准饲料对载脂蛋白E基因敲除小鼠(ApoE-/-)血液学及病理学的影响。方法 用西方膳食饲料和标准饲料对3月龄ApoE-/-小鼠进行喂养，并于喂养3个月、4个月和5个月时，采集小鼠外周血和主动脉弓。用全自动血液分析仪检测其血脂水平，并通过切片及染色，观察其动脉粥样硬化斑块的形成情况。结果 血脂方面，西方膳食及标准饲料喂养的小鼠血清中总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)比对照组C57小鼠明显升高，除了经4个月喂养的小鼠HDL-C外，其他各月龄血脂指标西方膳食喂养组都显著高于标准饲料组；在主动脉弓病理切片及染色方面，相同月龄下，西方膳食组小鼠主动脉斑块明显并且AS发病率明显升高。结论 西方膳食饲料及标准饲料均可诱导ApoE-/-小鼠表现AS病症，西方膳食饲料的诱导效果更明显。

The Effect of Western Diet on the Apolipoprotein E Gene Knock out Mice

Objective To study the effect of western diet and standard diet on the blood fat and aortic arch pathology of apolipoprotein E gene knock out mice. Method We feed 3 months old ApoE-/- mice with western dietary or standard diet for 3 months, 4 months and 5 months, respectively, then collect peripheral blood and aortic arch. We use Automatic blood analyzer to detect lipid level in serum, and observe the formation of atherosclerotic plaque through sectioning and staining.Result On the blood lipids detection, total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) in serum of mice feeding western diet and standard diet were significantly higher than the control group. In addition to HDL-C mice 4 months feeding, the lipid index of western diet lipid groups are significantly higher than the standard diet group on the same age. On the stained sections, western diet aortic plaque and incidence of atherosclerosis are significantly increased.Conclusion Both Western dietary and standard diet can induce ApoE-/- mice showed AS symptoms, the effect of western dietary is more obvious.