不同诱导条件对树鼩骨髓间充质干细胞向成骨细胞分化的影响

目的 探讨不同诱导条件对树鼩骨髓间充质干细胞（BM-MSCs）体外向成骨细胞分化的影响。方法 取 P3代树鼩骨髓间充质干细胞分成7组进行诱导。A组：高糖DMEM+1μmol/L地塞米松+100μmol/L维生素C+10 mmol/Lβ-磷酸甘油钠；B组：高糖 DMEM+50 ng/mL BMP-2；C组：高糖 DMEM+80 ng/mL BMP-2；D组：高糖DMEM+50μmol/L维生素C+10 mmol/Lβ-磷酸甘油钠+20ng/m L BMP-2；E组：高糖 DMEM+1μmol/L地塞米松 +100μmol/L维生素C+20 mmol/Lβ-磷酸甘油钠；F组：高糖DMEM+100μmol/L地塞米松+100μmol/L维生素 C+10 mmol/Lβ-磷酸甘油钠；G组：DMEM/F12+0.1μmol/L地塞米松 +50μmol/L维生素C+10 mmol/Lβ-磷酸甘油钠。诱导18 d后进行碱性磷酸酶和茜素红染色鉴定其诱导分化情况。结果 每一组碱性磷酸酶染色呈不同程度的阳性，茜素红染色可在A、B、C、D和E组观察到明显矿化结节。结论 A组、B组、C组、D组和 E组可以诱导树鼩BM-MSCs向成骨细胞分化，其中以A组和B组效果最为突出。

Effects Induced by Different Cond itions on the Differentiation of Tree Shrew Bone M arrow M esenchym al Stem Cells into Osteob lasts

Ob jective To investigate the effects of different mediums on the differentiation of tree shrew bone marrow mesenchymal stem cells（BM-MSCs） into osteoblasts in vitro.Method Tree shrew bone marrow mesenchymal stem cells（MSCs） were isolated and cultured in vitro by density gradient centrifugation and adherent culture.The tree shrew bonemarrow mesenchymal stem cells of P3 generations were divided into 7 groups.Group A：high glucose DMEM+1 μmol/L dexamethasone+100 μmol/L vitam in C+10 mmol/Lβ-glycerophosphate；Group B：high glucose DMEM+50 ng/m L BMP-2；Group C：high glucose DMEM+80 ng/m L BMP-2；Group D：high glucose DMEM+50μmol/L Vitam in C+10 mmol/Lβ -glycerophosphate+20 ng/m L BMP-2；Group E： high glucose DMEM+1 μmol/L dexamethasone+100 μmol/L Vitamin C+20 mmol/L βphosphoglyceride； Group F： high glucose DMEM+100 μmol/L dexamethasone+100 μmol/L Vitam in C+10 mmol/Lβ-phosphoglyceride；Group G：DMEM/F12+0.1μmol/L dexamethasone+50μmol/L Vitamin C+10 mmol/Lβ-phosphoglyceride.After 18 d，stain the cells with alkaline phosphatase and alizarin red.Result The result of each group of alkaline phosphatase staining were showed positive in different degree；the result of alizarin red staining in group A，B，C，D and group E were observed significantm ineral nodules.Conclusion Group A，group B，group C，group D and group E induced differentiation mediums could induced tree shrew BM-MSCs to differentiate into osteoblasts，among which group A and group B were the most effective.