高湿哮喘动物模型的建立

目的 研究高湿环境对哮喘的影响，建立高湿损伤动物模型和高湿哮喘动物模型。方法 雌性BALB/c小鼠置于人工气候箱中模拟高湿环境，分为对照组(A组)、哮喘组(B组)、高湿组(C组)和高湿哮喘组(D组)。A组置于常温常湿条件下饲养，B组在常温常湿环境下饲养，并通过卵清蛋白(OVA)致敏和激发，C组置于常温高湿环境下饲养，D组在常温高湿环境下饲养，并通过卵清蛋白(OVA)致敏和激发。结果 B、C、D组与A组相比，出现不同程度的粪便稀烂、皮毛暗淡、行为暴躁、体质量减轻、血常规指标异常(P<0.05)，B、D组免疫炎症反应、肺泡灌洗液嗜酸性细胞比例、肺部病理变化等情况明显高于A、C组(P<0.05)，且D组炎症反应高于B组(P<0.05)；结论 高湿环境可以诱导或者加剧哮喘症状，高湿哮喘动物模型可为在高湿环境中研究哮喘的发生、发展奠定基础。

Establishment of Asthma Animal Models in Damp Environment

Objective Studying the different effect of high humidity environment on healthy mice and asthma mice; establishing models of high humidity injury animals and asthma animals in high humidity environment. Method Female BALB/c mice were placed in artificial climate box to simulate a high humidity environment. The mice were divided into control group (group A), asthma group (group B), high humidity group (group C) and high humidity asthma group(group D). Group A was fed under normal temperature and normal humidity. The B group was fed under normal temperature and normal humidity and sensitized and stimulated by OVA. The C group was fed under normal temperature and high humidity. The D group was raised was fed under normal temperature and high humidity and sensitized and stimulated by ovalbumin (OVA). Result Compared with B, C, D group and A group, there are different degrees of pulpy stool, dark fur, irascible behavior, weight loss, abnormal blood routine index (P<0.05). The immune inflammatory response, the proportion of eosinophils in alveolar lavage fluid, and pathological changes in lung in group B and D were significantly higher than those in group A and C (P<0.05).The inflammatory response in group D was higher than that in group B (P<0.05). Conclusion The high humidity environment can induce or aggravate the symptoms of asthma. The animal model of asthma in high humidity environment can lay the foundation for the study of the occurrence and development of asthma in the high humidity environment.