抽取小鼠脑脊液简便易行的新方法

目的 建立抽取小鼠脑脊液的一种简单易行，材料低廉，生存率与成功率高的方法，为小鼠脑脊液的相关研究提供参考。方法 自制微量吸管。将小鼠头部固定与身体大约成120°角，对小鼠皮下组织和肌肉用镊子进行钝性分离，找到小鼠白色硬脊膜，进管抵达小脑延髓池，缓慢抽取小鼠脑脊液并将管退出，最后缝合小鼠伤口。结果成功获得小鼠脑脊液2～5 μL，小鼠存活。讨论 目前，大多文献记载的是大鼠脑脊液的抽取方法或较为复杂的小鼠脑脊液抽取方法，而此种针对小鼠的脑脊液抽取方法不但简单易行，对仪器要求低，成功率高而且小鼠生存率高，可以反复抽取。

A New Method is Simple and Easy to Extract the Cerebrospinal Fluid in Mice

Objective To establish a simple method for drawing out the CSF of mice and provide references for relative researches. Method First place the mouse in the position that the head forms 120°, shave the skin and separate the subcutaneous tissue and muscle bluntly. As a result , the dura mater of the cisterna magna appears clearly. Penetrate the white dura mater and then reach the cerebellomedullary cistern by the capillary tube. Obtain the CSF and withdraw the tube. Finally, suture the mouse. Result 2—5 μL CSF samples can be collected successfully with mice survived. Conclusion Compared with others, the method we established is proved to be with cheaper material, higher success rate and survival rate.